IgG antibody | R10Z8E9
Mouse anti Human IgG (CH2 Domain), clone R10Z8E9 recognises Human IgG.
Clone R10Z8E9 appears to be specific to Human IgG and been demonstrated by ELISA not to recognise IgG from Non-Human Primate species (Baboon, Marmoset, Rhesus and Cynomologus macaques), nor does it recognize mouse or rat immunoglobulin.
NB: This reagent may not be used for the detection of therapeutic antibodies from samples obtained from experimental animals without prior consent by the owner of EP 1 853 921 (F. Hoffmann-La Roche AG, Basel, Switzerland) and /or US 7,955,806 (HOFFMANN-LA ROCHE, INC., Nutley, NJ).
View a summary of anti-human IgG Fc specific antibodies for the detection of therapeutic antibodies.
Clone R10Z8E9 appears to be specific to Human IgG and been demonstrated by ELISA not to recognise IgG from Non-Human Primate species (Baboon, Marmoset, Rhesus and Cynomologus macaques), nor does it recognize mouse or rat immunoglobulin.
NB: This reagent may not be used for the detection of therapeutic antibodies from samples obtained from experimental animals without prior consent by the owner of EP 1 853 921 (F. Hoffmann-La Roche AG, Basel, Switzerland) and /or US 7,955,806 (HOFFMANN-LA ROCHE, INC., Nutley, NJ).
View a summary of anti-human IgG Fc specific antibodies for the detection of therapeutic antibodies.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Cynomolgus monkey Baboon Marmoset Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Polyclonal Human IgG
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P01857 Related reagents
- P01859 Related reagents
- P01859 Related reagents
- Entrez Gene
- IGHG1 Related reagents
- IGHG2 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0006955 immune response
- Licensed Use
- Not for the detection of therapeutic antibodies from samples obtained from experimental animals without prior consent by the owner of EP 1 853 921 (F. Hoffmann-La Roche AG, Basel, Switzerland) and /or US 7,955,806 (HOFFMANN-LA ROCHE, INC., Nutley, NJ)
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product is suitable for use in indirect ELISA applications.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | 0.5 mg | E WB | ||
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | 1 ml | F | ||
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | 1 mg | C E P RE WB | ||
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | 1 mg | F |
Product Specific References
References for IgG antibody
-
Stubenrauch, K. et al. (2009) Evaluation of an immunoassay for human-specific quantitation of therapeutic antibodies in serum samples from non-human primates.
J Pharm Biomed Anal. 49 (4): 1003-8.
