IgG antibody | R10Z8E9

Mouse anti Human IgG (CH2 Domain)

Product Type
Monoclonal Antibody
Clone
R10Z8E9
Isotype
IgG1
Specificity
IgG
Region
(CH2 Domain)

Product Code Applications Pack Size List Price Your Price Qty
MCA5748G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human IgG (CH2 Domain), clone R10Z8E9 recognises Human IgG.

Clone R10Z8E9 appears to be specific to Human IgG and been demonstrated by ELISA not to recognise IgG from Non-Human Primate species (Baboon, Marmoset, Rhesus and Cynomologus macaques), nor does it recognize mouse or rat immunoglobulin.

NB: This reagent may not be used for the detection of therapeutic antibodies from samples obtained from experimental animals without prior consent by the owner of EP 1 853 921 (F. Hoffmann-La Roche AG, Basel, Switzerland) and /or US 7,955,806 (HOFFMANN-LA ROCHE, INC., Nutley, NJ).

View a summary of anti-human IgG Fc specific antibodies for the detection of therapeutic antibodies.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
Cynomolgus monkey
Baboon
Marmoset
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Polyclonal Human IgG
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product is suitable for use in indirect ELISA applications.

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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for IgG antibody

  1. Stubenrauch, K. et al. (2009) Evaluation of an immunoassay for human-specific quantitation of therapeutic antibodies in serum samples from non-human primates.
    J Pharm Biomed Anal. 49 (4): 1003-8.
  2. Gilchuk, P. et al. (2018) Efficacy of Human Monoclonal Antibody Monotherapy Against Bundibugyo Virus Infection in Nonhuman Primates.
    J Infect Dis. 218 (suppl_5): S565-S573.
  3. Cross, R.W. et al. (2020) Prior vaccination with rVSV-ZEBOV does not interfere with but improves efficacy of postexposure antibody treatment.
    Nat Commun. 11 (1): 3736.
  4. Gilchuk, P. et al. (2020) Analysis of a Therapeutic Antibody Cocktail Reveals Determinants for Cooperative and Broad Ebolavirus Neutralization.
    Immunity. 52 (2): 388-403.e12.

RRID
AB_10965068
UniProt
P01857
P01859
P01859
Entrez Gene
IGHG1
IGHG2
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response
Licensed Use
Not for the detection of therapeutic antibodies from samples obtained from experimental animals without prior consent by the owner of EP 1 853 921 (F. Hoffmann-La Roche AG, Basel, Switzerland) and /or US 7,955,806 (HOFFMANN-LA ROCHE, INC., Nutley, NJ)
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products