IgG antibody | R10Z8E9
Mouse anti Human IgG (CH2 Domain), clone R10Z8E9 (MCA5748G) used as a coating antibosy in a sandwich ELISA to evaluate circulating therapeutic antibody levels in a macaque disease model.
Image caption:
Circulating host and therapeutic antibodies in EBOV-challenged and treated rhesus macaques.
Circulating MIL77 concentrations for the rVSV-EBOV + MIL77 and MIL77 groups are represented over time (j). rVSV-EBOV + MIL77 (n = 5), rVSV-EBOV (n = 5), MIL77 (n = 5), control (n = 1). Significance of concentration differences between rVSV-EBOV + MIL77 and MIL77 only groups (***p <0.001) were determined with two-tailed t-tests using Holm–Sidak correction for multiple comparisons.
From: Cross RW, Bornholdt ZA, Prasad AN, Geisbert JB, Borisevich V, Agans KN, Deer DJ, Melody K, Fenton KA, Feldmann H, Sprecher A, Zeitlin L, Geisbert TW.
Prior vaccination with rVSV-ZEBOV does not interfere with but improves efficacy of postexposure antibody treatment.
Nat Commun. 2020 Jul 27;11(1):3736.
doi: 10.1038/s41467-020-17446-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
E ResetMouse anti Human IgG (CH2 Domain)
- Product Type
- Monoclonal Antibody
- Clone
- R10Z8E9
- Isotype
- IgG1
- Specificity
- IgG
- Region
- (CH2 Domain)
Antibody Quality and Performance Guarantee
| Mouse anti Human IgG (CH2 Domain), clone R10Z8E9 recognises Human IgG. Clone R10Z8E9 appears to be specific to Human IgG and been demonstrated by ELISA not to recognise IgG from Non-Human Primate species (Baboon, Marmoset, Rhesus and Cynomologus macaques), nor does it recognize mouse or rat immunoglobulin. NB: This reagent may not be used for the detection of therapeutic antibodies from samples obtained from experimental animals without prior consent by the owner of EP 1 853 921 (F. Hoffmann-La Roche AG, Basel, Switzerland) and /or US 7,955,806 (HOFFMANN-LA ROCHE, INC., Nutley, NJ). View a summary of anti-human IgG Fc specific antibodies for the detection of therapeutic antibodies. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Cynomolgus monkey Baboon Marmoset Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Polyclonal Human IgG
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product is suitable for use in indirect ELISA applications.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
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| Description | Rabbit F(ab')2 anti Mouse IgG:RPE | ||||||
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
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| Description | Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
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| Description | Rabbit F(ab')2 anti Mouse IgG:FITC | ||||||
References for IgG antibody
-
Stubenrauch, K. et al. (2009) Evaluation of an immunoassay for human-specific quantitation of therapeutic antibodies in serum samples from non-human primates.
J Pharm Biomed Anal. 49 (4): 1003-8. -
Gilchuk, P. et al. (2018) Efficacy of Human Monoclonal Antibody Monotherapy Against Bundibugyo Virus Infection in Nonhuman Primates.
J Infect Dis. 218 (suppl_5): S565-S573. -
Cross, R.W. et al. (2020) Prior vaccination with rVSV-ZEBOV does not interfere with but improves efficacy of postexposure antibody treatment.
Nat Commun. 11 (1): 3736. -
Gilchuk, P. et al. (2020) Analysis of a Therapeutic Antibody Cocktail Reveals Determinants for Cooperative and Broad Ebolavirus Neutralization.
Immunity. 52 (2): 388-403.e12.
- RRID
- AB_10965068
- UniProt
- P01857
- P01859
- P01859
- Entrez Gene
- IGHG1
- IGHG2
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0006955 immune response
- Licensed Use
- Not for the detection of therapeutic antibodies from samples obtained from experimental animals without prior consent by the owner of EP 1 853 921 (F. Hoffmann-La Roche AG, Basel, Switzerland) and /or US 7,955,806 (HOFFMANN-LA ROCHE, INC., Nutley, NJ)
View more products with IGG specificity
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