IgG antibody | R10Z8E9

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Mouse anti Human IgG (CH2 Domain)

Product Type
Monoclonal Antibody
Clone
R10Z8E9
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5748G E 0.2 mg
Mouse anti Human IgG (CH2 Domain), clone R10Z8E9 recognises Human IgG.

Clone R10Z8E9 appears to be specific to Human IgG and been demonstrated by ELISA not to recognise IgG from Non-Human Primate species (Baboon, Marmoset, Rhesus and Cynomologus macaques), nor does it recognize mouse or rat immunoglobulin.

NB: This reagent may not be used for the detection of therapeutic antibodies from samples obtained from experimental animals without prior consent by the owner of EP 1 853 921 (F. Hoffmann-La Roche AG, Basel, Switzerland) and /or US 7,955,806 (HOFFMANN-LA ROCHE, INC., Nutley, NJ).

View a summary of anti-human IgG Fc specific antibodies for the detection of therapeutic antibodies.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
Cynomolgus monkey
Baboon
Marmoset
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Polyclonal Human IgG
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01857 Related reagents
P01859 Related reagents
P01859 Related reagents
Entrez Gene
IGHG1 Related reagents
IGHG2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response
Licensed Use
Not for the detection of therapeutic antibodies from samples obtained from experimental animals without prior consent by the owner of EP 1 853 921 (F. Hoffmann-La Roche AG, Basel, Switzerland) and /or US 7,955,806 (HOFFMANN-LA ROCHE, INC., Nutley, NJ)
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product is suitable for use in indirect ELISA applications.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for IgG antibody

  1. Stubenrauch, K. et al. (2009) Evaluation of an immunoassay for human-specific quantitation of therapeutic antibodies in serum samples from non-human primates.
    J Pharm Biomed Anal. 49 (4): 1003-8.