HSF1 antibody | CD01/1B5

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Mouse anti HSF1

Product Type
PrecisionAb Monoclonal
Clone
CD01/1B5
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00893
Datasheet
IP WB
SDS
100 µl loader

Mouse anti HSF1 antibody, clone CD01/1B5 recognizes heat shock factor protein 1.

HSF1 is a stress-induced DNA-binding transcription factor that plays a critical role in the heat shock response, protecting cells from cellular damage. In unstressed cells, HSF1 is found in a chaperone complex that maintains it in a non-DNA-binding form. Under conditions of temperature stress or other environmental perturbations, HSF1 translocates from the cytosol to the nucleus where it promotes transcription of heat shock proteins (HSPs) (Li et al. 2017). HSF1 is constitutively activated in malignant cells, and cancer cells become dependent on HSF1 for growth and survival. Furthermore, high levels of HSF1 have been found to correlate with poor clinical outcomes in some cancer types (Carpenter et al. 2017). HSF1 has been suggested as a target for cancer therapy (Dai 2018).

Product Details

Target Species
Human
Western Blotting
Mouse anti HSF1 antibody detects a band of approximately 75 kDa in HeLa cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-529 of human HSF1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q00613
Entrez Gene
HSF1
GO Terms
GO:0005515 protein binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005737 cytoplasm
GO:0006355 regulation of transcription, DNA-dependent
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of HSF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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