Hexokinase 1 antibody | 7A7

Mouse anti Hexokinase 1

Product Type
PrecisionAb Monoclonal
Clone
7A7
Isotype
IgG1
Format
Purified
Specificity
Hexokinase 1

Product Code Applications Pack Size List Price Your Price Qty
VMA00230
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human hexokinase 1 antibody recognizes hexokinase 1, also known as HK I, brain form hexokinase, glycolytic enzyme and hexokinase type I.

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Encoded by the HK1 gene, hexokinase 1 represents a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in HK1 have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of HK1 results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein (provided by RefSeq, Apr 2009).

Mouse anti Human hexokinase 1 antibody detects a band of 102 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti hexokinase 1 detects a band of approximately 102 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Purified recombinant fragment of human hexokinase 1 expressed in E. coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P19367
Entrez Gene
HK1
GO Terms
GO:0004340 glucokinase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005741 mitochondrial outer membrane
GO:0006096 glycolysis
GO:0015758 glucose transport
GO:0055085 transmembrane transport
View more products with Hexokinase 1 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products