HbA1c antibody | AbD15785

Human anti Human HbA1c

Product Type
Monoclonal Antibody
Clone
AbD15785
Isotype
HuCAL Fab monovalent
Specificity
HbA1c

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HCA180
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E 0.1 mg loader
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Human anti Human HbA1c antibody, clone AbD15785 recognizes HbA1c, i.e. hemoglobin when glycated at the N-terminal valine of the hemoglobin β chain. It does not bind to non-glycated hemoglobin. Besides binding to the normal glycated hemoglobin, it also recognizes the glycated hemoglobin variants HbC, HbE, HbD and to a lesser degree HbS. Elevated HbF cannot be detected due to the absence of the hemoglobin β chain.

The measurement of HbA1c levels is used to identify the average plasma glucose concentration. In Diabetes mellitus, higher levels of HbA1c have been associated with cardiovascular disease and other diseases. Levels of more than 6.5% HbA1c (48 mmol/mol) are used as a criterion to diagnose diabetes.

Target Species
Human
Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
StrepTactin affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
The immunogens used in the generation of this antibody were:
A glycated peptide, corresponding to N-terminal 9 amino acids of haemoglobin beta chain coupled to carrier proteins
Purified HbA1c
Affinity
The affinity of this antibody, measured against the immunogen, was measured as KD=28 nM by real time, label-free molecular interaction analysis using HbA1c-BSA and HbA1c-TRF conjugates as immobilized antigen.

The affinity of this antibody, as measured against purified HbA1c, was measured as KD=65 nM by real time, label-free molecular interaction analysis using full length HbA1c as immobilized antigen.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product is suitable for use in direct and indirect ELISA applications.

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Description Mouse anti V5-Tag:Alk. Phos.
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Description Mouse anti V5-Tag:Biotin
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Description Mouse anti V5-Tag:HRP
Mouse anti Strep-Tag Classic:HRP MCA2489P E WB 75 µl loader
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Description Mouse anti Strep-Tag Classic:HRP

Synonyms
Glycated HbC
Glycated HbS
Glycated Hemoglobin
Hb1c
Hemoglobin A1c
UniProt
P68871
Entrez Gene
HBB
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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