HbA1c antibody | AbD15785

100% Secure


Human anti Human HbA1c

Product Type
Monoclonal Antibody
Clone
AbD15785
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA180 E 0.1 mg
Human anti Human HbA1c antibody, clone AbD15785 recognizes HbA1c, i.e. hemoglobin when glycated at the N-terminal valine of the hemoglobin β chain. It does not bind to non-glycated hemoglobin. Besides binding to the normal glycated hemoglobin, it also recognizes the glycated hemoglobin variants HbC, HbE, HbD and to a lesser degree HbS. Elevated HbF cannot be detected due to the absence of the hemoglobin β chain.

The measurement of HbA1c levels is used to identify the average plasma glucose concentration. In Diabetes mellitus, higher levels of HbA1c have been associated with cardiovascular disease and other diseases. Levels of more than 6.5% HbA1c (48 mmol/mol) are used as a criterion to diagnose diabetes.

Product Details

Target Species
Human
Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
StrepTactin affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
The immunogens used in the generation of this antibody were:
A glycated peptide, corresponding to N-terminal 9 amino acids of haemoglobin beta chain coupled to carrier proteins
Purified HbA1c
Affinity
The affinity of this antibody, measured against the immunogen, was measured as KD=28 nM by real time, label-free molecular interaction analysis using HbA1c-BSA and HbA1c-TRF conjugates as immobilized antigen.

The affinity of this antibody, as measured against purified HbA1c, was measured as KD=65 nM by real time, label-free molecular interaction analysis using full length HbA1c as immobilized antigen.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P68871 Related reagents
Entrez Gene
HBB Related reagents
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of HbA1c antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product is suitable for use in direct and indirect ELISA applications.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti V5-Tag:Alk. Phos. MCA1360A 0.1 mg E WB
Mouse anti V5-Tag:Biotin MCA1360B 0.1 mg C E WB
Mouse anti V5-Tag:HRP MCA1360P 0.1 mg E WB
Mouse anti Strep-Tag Classic:HRP MCA2489P 75 µl E WB

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
HuCAL Fab-V5Sx2 Negative Control HCA125 0.1 mg C E F P WB