GFAP antibody | RM246
Rabbit anti Human GFAP antibody, clone RM246 (MCA6305) used to label astroglial cells in rat hippocampus by immunofluorescence.
Image caption:
Cerebrolysin decreases the density of glial cells after pilocarpine-induced seizure. The administration of cerebrolysin decreased the density of glial cells after pilocarpine-induced seizure. (A,D) Iba-1 (red), GFAP (green), and DAPI (blue) in the hippocampal CA1 (A) and CA3 (D) regions. The administration of cerebrolysin after a seizure decreased the density of glial cells in the hippocampal CA1 and CA3 regions compared to the seizure-vehicle groups. Scale bar = 20 μm. (B,C,E,F) A graph of the density of glial cells according to the standard. The data are the mean ± SEM, n = 5, from each sham group; n = 5–7 for each seizure group. *p <0.05 vs. vehicle-treated group; #p <0.05 vs. sham-operated group [Kruskal–Wallis test with post hoc test: (B) Chi square = 17.244, df = 3, p = 0.001, (C) Chi square = 17.153, df = 3, p <0.001, (E) Chi square = 17.456, df = 3, p = 0.001, (F) Chi square = 18.292, df = 3, p < 0.001].
From: Kang DH, Choi BY, Lee SH, Kho AR, Jeong JH, Hong DK, Kang BS, Park MK, Song HK, Choi HC, Lim MS, Suh SW.
Effects of Cerebrolysin on Hippocampal Neuronal Death After Pilocarpine-Induced Seizure.
Front Neurosci. 2020 Oct 16;14:568813.
doi: 10.3389/fnins.2020.568813.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Human GFAP antibody, clone RM246 (MCA6305) used to label astroglial cells in rat hippocampus by immunofluorescence.
Image caption:
Cerebrolysin decreases the density of glial cells after pilocarpine-induced seizure. The administration of cerebrolysin decreased the density of glial cells after pilocarpine-induced seizure. (A,D) Iba-1 (red), GFAP (green), and DAPI (blue) in the hippocampal CA1 (A) and CA3 (D) regions. The administration of cerebrolysin after a seizure decreased the density of glial cells in the hippocampal CA1 and CA3 regions compared to the seizure-vehicle groups. Scale bar = 20 μm. (B,C,E,F) A graph of the density of glial cells according to the standard. The data are the mean ± SEM, n = 5, from each sham group; n = 5–7 for each seizure group. *p <0.05 vs. vehicle-treated group; #p <0.05 vs. sham-operated group [Kruskal–Wallis test with post hoc test: (B) Chi square = 17.244, df = 3, p = 0.001, (C) Chi square = 17.153, df = 3, p <0.001, (E) Chi square = 17.456, df = 3, p = 0.001, (F) Chi square = 18.292, df = 3, p < 0.001].
From: Kang DH, Choi BY, Lee SH, Kho AR, Jeong JH, Hong DK, Kang BS, Park MK, Song HK, Choi HC, Lim MS, Suh SW.
Effects of Cerebrolysin on Hippocampal Neuronal Death After Pilocarpine-Induced Seizure.
Front Neurosci. 2020 Oct 16;14:568813.
doi: 10.3389/fnins.2020.568813.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti GFAP
- Product Type
- Monoclonal Antibody
- Clone
- RM246
- Isotype
- IgG
- Specificity
- GFAP
Antibody Quality and Performance Guarantee
| Rabbit anti Human GFAP antibody, clone RM246 recognizes glial fibrillary acidic protein (GFAP). GFAP plays a role in several cellular functions within the central nervous system (CNS), including cell structure and stability, communication, motility and mitosis, and is rapidly synthesized during astrogliosis following trauma/injury (Eng and Ghirnikar 1994). GFAP is an intermediate filament protein that is primarily expressed in astrocytes, and is upregulated following injury to the central nervous system (CNS) (Brenner et al. 2014), inhibiting the inflammatory response and limiting damage within a controllable region (Zhang et al. 2017). Dominant acting mutations in GFAP leading to elevated GFAP levels in astrocytes is characteristic of Alexander disease, a fatal neurological illness (Messing et al. 2019). GFAP is also a potential marker for tumors with cartilaginous differentiation (Santos et al. 2009). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- A peptide corresponding to the N-terminus of Human GFAP.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin |  |
1/200 | 1/500 |
| Western Blotting | |
1/1000 | 1/3000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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References for GFAP antibody
-
Despa, F. et al. (2020) Amylin as a Potential Link between Type 2 Diabetes and Alzheimer Disease.
Ann Neurol. 87 (3): 486. -
Kang, D.H. et al. (2020) Effects of Cerebrolysin on Hippocampal Neuronal Death After Pilocarpine-Induced Seizure.
Front Neurosci. 14: 568813.
MCA6305
155778
165648
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