Galectin-3 Binding Protein antibody | OTI2B1

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Anti Galectin-3 Binding Protein Antibody, clone OTI2B1 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with galectin-3-binding protein antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 120 second exposure. Arrow points to galectin-3-binding protein (molecular weight 75 kDa)

Mouse anti Galectin-3 Binding Protein

Galectin-3 binding protein antibody recognizes galectin-3-binding protein, also known as L3 antigen, LGALS3BP, MAC2BP, Mac-2-binding protein, basement membrane autoantigen p105, lectin galactoside-binding soluble 3-binding protein, or Mac-2 BP.

Galectin-3 Binding Protein Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI2B1
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00292	WB	100 µl
VMA00292KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human galectin-3-binding protein antibody recognizes the galectin-3-binding protein, also known as L3 antigen, MAC2BP, Mac-2-binding protein, basement membrane autoantigen p105, lectin galactoside-binding soluble 3-binding protein, mac-2 BP, serum protein 90K, transport and golgi organization 10 homolog B, and tumor-associated antigen 90K.

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Using fluorescence in situ hybridization the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin-1 (provided by RefSeq, Jul 2008).

Mouse anti Human galectin-3-binding protein antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti galectin-3 binding protein detects a band of approximately 75 kDa in A431 cell lysates
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant.
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein corresponding to amino acids 19-300 of human galectin-3-binding protein (NP_005558) produced in E. coli
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg A431 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.

More Information

UniProt: Q08380 Related reagents
Entrez Gene: LGALS3BP Related reagents
GO Terms: GO:0005515 protein binding; GO:0016020 membrane; GO:0007155 cell adhesion; GO:0005044 scavenger receptor activity; GO:0005578 proteinaceous extracellular matrix; GO:0005615 extracellular space; GO:0006968 cellular defense response
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Galectin-3 Binding Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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