Ezrin antibody

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Mouse anti Ezrin

Product Type
PrecisionAb Monoclonal
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00344
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human ezrin antibody recognizes human ezrin, otherwise known as p81 or villin 2.

Ezrin is a 586 amino acid, cytoplasmic peripheral membrane protein containing a single FERM domain and member of the ezrin, radixin, moesin (ERM) protein family, which acts as protein-tyrosine kinase substrate. Ezrin is enriched in microvilli and other cell surface structures and plays a key role in cell surface structure adhesion, membrane trafficking, motility and organization (Ng et al. 2001). Ezrin acts as a linker protein between the plasma membrane and the actin cytoskeleton, and provides anchorage for cell surface molecules such as ICAM and CD44 (Finnerty et al. 2004). Studies have implicated ezrin in the metastatic capacity of various types of human tumors including breast, prostate, bone, endometrial and colon cancers (Ohtani et al. 1999, Elzaqheid et al. 2008).

Mouse anti Human ezrin antibody recognizes ezrin as a single band of 80 kDa in multiple human cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti ezrin detects a band of approximately 80 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human ezrin

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P15311 Related reagents
Entrez Gene
EZR Related reagents
GO Terms
GO:0051015 actin filament binding
GO:0051017 actin filament bundle assembly
GO:0050839 cell adhesion molecule binding
GO:0005829 cytosol
GO:0005730 nucleolus
GO:0005884 actin filament
GO:0007016 cytoskeletal anchoring at plasma membrane
GO:0007159 leukocyte cell-cell adhesion
GO:0007411 axon guidance
GO:0008360 regulation of cell shape
GO:0016324 apical plasma membrane
GO:0019898 extrinsic to membrane
GO:0022614 membrane to membrane docking
GO:0030175 filopodium
GO:0030863 cortical cytoskeleton
GO:0031528 microvillus membrane
GO:0032587 ruffle membrane
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Ezrin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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