Estrogen Receptor Alpha antibody | RM292

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Rabbit anti Estrogen Receptor Alpha

Product Type
Monoclonal Antibody
Clone
RM292
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6322
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti Human estrogen receptor alpha antibody recognizes estrogen receptor (ER), also known as Estradiol receptor, ESR1, NR3A1. Estrogen is a steroid hormone which, among other functions, regulates the growth, development, and physiology of the human reproductive system. Estrogen is synthesized by ovaries and by cells expressing the enzyme aromatase, and recognized by cells expressing the estrogen receptor (Hewitt and Korach 2018). Estrogen receptors are nuclear receptors which function as transcription factors, switching on expression of genes involved with reproductive organ development, bone modeling, function of the cardiovascular system, metabolism, and behaviour (Lee et al. 2012). Abnormal expression of estrogen receptors is involved with initiation and development of hormone-related cancers, and can also affect the outcome of cancer therapy. Around 70% of breast cancers express the estrogen receptor, and for these patients, antiestrogen therapy is mainstay treatment (Drago et al. 2019). Estrogen and the estrogen receptor also appear to be involved with lung carcinogenesis (Hsu et al. 2017).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to residues near the N-terminus of Human estrogen receptor alpha

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

UniProt
P03372
Entrez Gene
ESR1
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005496 steroid binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0008013 beta-catenin binding
GO:0008270 zinc ion binding
GO:0016585 chromatin remodeling complex
GO:0019899 enzyme binding
GO:0030235 nitric-oxide synthase regulator activity
GO:0030284 estrogen receptor activity
GO:0032355 response to estradiol stimulus
GO:0034056 estrogen response element binding
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0048386 positive regulation of retinoic acid receptor signaling pathway
Regulatory
For research purposes only

Applications of Estrogen Receptor Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/100 1/200
Western Blotting 1/200 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

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