EMR3 antibody | 3D7

Hamster anti Human EMR3

Product Type
Monoclonal Antibody
Clone
3D7
Isotype
IgG
Specificity
EMR3

Product Code Applications Pack Size List Price Your Price Qty
MCA2476GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Hamster anti Human EMR3 antibody, clone 3D7 recognizes human epidermal growth factor (EGF) module-containing mucin-like hormone receptor 3 (EMR3), a ~56 kDa member of the EGF-7 transmembrane (TM7) family of adhesion receptors. EMR3 is expressed at the cell surface as a heterodimer.

EMR3 is predominantly expressed on granulocytes, and at lower levels on mature myeloid cells, monocytes and dendritic cells. EMR3 is absent on lymphocytes, haematopoietic stem cells and myeloid progenitors. EMR3 is up-regulated during late stages of neutrophil differentiation and is a marker for terminally differentiated cells (Matmati et al.2007).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
ARHO-EMR3-CD97 (EGF1) transfectants.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized armenian hamster were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat F(ab')2 anti Hamster IgG:Dylight®800
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
List Price Your Price
loader
Description Goat F(ab')2 anti Hamster IgG:FITC
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
List Price Your Price
loader
Description Goat anti Hamster IgG:Biotin
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
List Price Your Price
loader
Description Goat anti Hamster IgG:FITC
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader
List Price Your Price
loader
Description Goat anti Hamster IgG:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader
List Price Your Price
loader
Description Hamster (Armenian) IgG Negative Control

References for EMR3 antibody

  1. Matmati, M. et al. (2007) The human EGF-TM7 receptor EMR3 is a marker for mature granulocytes.
    J Leukoc Biol. 81 (2): 440-8.
  2. Drewniak, A. etal. (2009) Changes in gene expression of granulocytes during in vivo granulocyte colony-stimulating factor/dexamethasone mobilization for transfusion purposes.
    Blood. 113: 5979-98.
  3. van de Geer, A. et al. (2017) Characterization of buffy coat-derived granulocytes for clinical use: a comparison with granulocyte colony-stimulating factor/dexamethasone-pretreated donor-derived products.
    Vox Sang. 112 (2): 173-82.
  4. Gazendam, R.P. et al. (2016) Impaired killing of Candida albicans. by granulocytes mobilized for transfusion purposes: a role for granule components.
    Haematologica. 101 (5): 587-96.

Further Reading

  1. Carpenter, G. & Cohen, S. (1990) Epidermal growth factor.
    J Biol Chem. 265 (14): 7709-12.

RRID
AB_872003
UniProt
Q9BY15
Entrez Gene
EMR3
GO Terms
GO:0005886 plasma membrane
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0004930 G-protein coupled receptor activity
GO:0005615 extracellular space
GO:0007218 neuropeptide signaling pathway
Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products