EGF R antibody | ICR10
Rat anti Human EGF Receptor
- Product Type
- Monoclonal Antibody
- Clone
- ICR10
- Isotype
- IgG2a
- Specificity
- EGF R
Antibody Quality and Performance Guarantee
| Rat anti Human EGF Receptor antibody, clone ICR10 recognizes the human epidermal growth factor receptor (EGF-R), which is over expressed in a high proportion of breast cancer cells and in a range of other carcinomas. High level expression of EGFR is often associated with advanced disease and poor prognosis. Rat anti Human EGF Receptor antibody, clone ICR10 binds to epitope B from EGFR (Lottaz et al. 2010 and Modjtahedi et al. 1993) and has an affinity of 6.7 x 10-9 M. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Extracellular domain of human EGF-receptor from head and neck carcinoma.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
||
| Immunoprecipitation | |
||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
- Histology Positive Control Tissue
- Human breast carcinoma
References for EGF R antibody
-
Modjtahedi, H. et al. (1993) Antitumor activity of combinations of antibodies directed against different epitopes on the extracellular domain of the human EGF receptor.
Cell Biophys. 22 (1-3): 129-46. -
Gilcrease, M.Z. et al. (2009) Alpha6beta4 integrin crosslinking induces EGFR clustering and promotes EGF-mediated Rho activation in breast cancer.
J Exp Clin Cancer Res. 28: 67. -
Lottaz,C. et al. (2010) Transcriptional Profiles of CD133+ and CD133- Glioblastoma-Derived Cancer Stem Cell Lines Suggest Different Cells of Origin
Cancer Res. 70: 2030-40. -
Modjtahedi, H. et al. (2012) Immunohistochemical discrimination of wild-type EGFR from EGFRvIII in fixed tumour specimens using anti-EGFR mAbs ICR9 and ICR10.
Br J Cancer. 106 (5): 883-8. -
Grinberg, O. et al. (2013) Antibody modified Bovine Serum Albumin microspheres for targeted delivery of anticancer agent Gemcitabine
Polymers for Advanced Technologies. 24 (3): 294-299. -
Khelwatty, S.A. et al. (2015) Acquired resistance to anti-EGFR mAb ICR62 in cancer cells is accompanied by an increased EGFR expression, HER-2/HER-3 signalling and sensitivity to pan HER blockers.
Br J Cancer. 113 (7): 1010-9. -
Tilburgs, T. et al. (2015) The HLA-G cycle provides for both NK tolerance and immunity at the maternal-fetal interface.
Proc Natl Acad Sci U S A. 112 (43): 13312-7. -
Tilburgs, T. et al. (2015) Human HLA-G+ extravillous trophoblasts: Immune-activating cells that interact with decidual leukocytes.
Proc Natl Acad Sci U S A. 112 (23): 7219-24.
View The Latest Product References
Further Reading
-
Modjtahedi, H. & Dean, C. (1994) The receptor for EGF and its ligands - expression, prognostic value and target for therapy in cancer (review).
Int J Oncol. 4 (2): 277-96.
MCA1784
147834
155651
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