EGF R antibody | ICR10

Rat anti Human EGF Receptor

Product Type
Monoclonal Antibody
Clone
ICR10
Isotype
IgG2a
Specificity
EGF R

Product Code Applications Pack Size List Price Your Price Qty
MCA1784
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP P 0.2 mg loader
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Rat anti Human EGF Receptor antibody, clone ICR10 recognizes the human epidermal growth factor receptor (EGF-R), which is over expressed in a high proportion of breast cancer cells and in a range of other carcinomas.

High level expression of EGFR is often associated with advanced disease and poor prognosis.

Rat anti Human EGF Receptor antibody, clone ICR10 binds to epitope B from EGFR (Lottaz et al. 2010 and Modjtahedi et al. 1993) and has an affinity of 6.7 x 10-9 M.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Extracellular domain of human EGF-receptor from head and neck carcinoma.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl
Histology Positive Control Tissue
Human breast carcinoma

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References for EGF R antibody

  1. Modjtahedi, H. & Dean, C. (1994) The receptor for EGF and its ligands - expression, prognostic value and target for therapy in cancer (review).
    Int J Oncol. 4 (2): 277-96.
  2. Lottaz,C. et al. (2010) Transcriptional Profiles of CD133+ and CD133- Glioblastoma-Derived Cancer Stem Cell Lines Suggest Different Cells of Origin
    Cancer Res. 70: 2030-40.
  3. Modjtahedi, H. et al. (1993) Antitumor activity of combinations of antibodies directed against different epitopes on the extracellular domain of the human EGF receptor.
    Cell Biophys. 22 (1-3): 129-46.
  4. Modjtahedi, H. et al. (2012) Immunohistochemical discrimination of wild-type EGFR from EGFRvIII in fixed tumour specimens using anti-EGFR mAbs ICR9 and ICR10.
    Br J Cancer. 106 (5): 883-8.
  5. Grinberg, O. et al. (2013) Antibody modified Bovine Serum Albumin microspheres for targeted delivery of anticancer agent Gemcitabine
    Polymers for Advanced Technologies. 24 (3): 294-299.
  6. Gilcrease, M.Z. et al. (2009) Alpha6beta4 integrin crosslinking induces EGFR clustering and promotes EGF-mediated Rho activation in breast cancer.
    J Exp Clin Cancer Res. 28: 67.
  7. Tilburgs, T. et al. (2015) Human HLA-G+ extravillous trophoblasts: Immune-activating cells that interact with decidual leukocytes.
    Proc Natl Acad Sci U S A. 112 (23): 7219-24.
  8. Tilburgs, T. et al. (2015) The HLA-G cycle provides for both NK tolerance and immunity at the maternal-fetal interface.
    Proc Natl Acad Sci U S A. 112 (43): 13312-7.
  9. View The Latest Product References
  10. Khelwatty, S.A. et al. (2015) Acquired resistance to anti-EGFR mAb ICR62 in cancer cells is accompanied by an increased EGFR expression, HER-2/HER-3 signalling and sensitivity to pan HER blockers.
    Br J Cancer. 113 (7): 1010-9.

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Synonyms
Epidermal Growth Factor Receptor
RRID
AB_322787
UniProt
P00533
Entrez Gene
EGFR
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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