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HER2/neu antibody | ICR55

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Anti Human HER2/neu Antibody, clone ICR55 thumbnail image 1
Anti Human HER2/neu Antibody, clone ICR55 gallery image 1
Figure A. PE conjugated Mouse anti Human EGFR (MCA1784PE) and Alexa Fluor 488 conjugated Rat IgG2a isotype control (MCA6005A488). Figure B. PE conjugated Mouse anti Human EGFR (MCA1784PE) and Alexa Fluor 488 conjugated Rat anti Human HER2 (MCA1788A488). All experiments performed on A431 cells gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer

Rat anti Human HER2/neu:Alexa Fluor®488

Product Type
Monoclonal Antibody
Clone
ICR55
Isotype
IgG2a
Specificity
HER2/neu

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Product Code Applications Pack Size List Price Your Price Qty
MCA1788A488
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SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Rat anti Human HER2/neu antibody, clone ICR55 recognizes epitope E of human HER2/neu, otherwise known as CD340 and c-ErbB-2, a proto-oncogene expressed at the cell surface of a range of tumor cells, often associated with poor prognosis, and an increased risk of metastasis.

HER2/neu is used in conjunction with antibodies recognizing both Estrogen and Progesterone receptors, for the classification of breast cancer tumors, and to determine patient prognosis and course of treatment.

Excessive HER2/neu is present in around 20% to 25% of invasive breast cancers, referred to as HER2-positive cancer, and can help determine whether drugs such as trastuzumab (Herceptin) or lapatinib (Tykerb), might be beneficial.

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®488 495 519
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Human Seroblock BUF070B F 200 Test
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References for HER2/neu antibody

  1. Dean, C.J. et al. (1993) Rat MAbs to the product of the c-erbB-2 proto-oncogene for diagnosis and therapy in breast cancer.
    Cell Biophys. 22 (1-3): 111-27.
  2. Shah, A.J. et al. (2014) Non-invasive molecular profiling of cancer using photoacoustic imaging of functionalized gold nanorods
    Proc. of SPIE Vol. 8943, 89435G

Further Reading

  1. Styles, J.M. et al. (1990) Rat monoclonal antibodies to the external domain of the product of the C-erbB-2 proto-oncogene.
    Int J Cancer. 45 (2): 320-4.

Synonyms
CD340
UniProt
P04626
Entrez Gene
ERBB2
GO Terms
GO:0003677 DNA binding
GO:0016021 integral to membrane
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0005006 epidermal growth factor receptor activity
GO:0043125 ErbB-3 class receptor binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006350 transcription
GO:0007507 heart development
GO:0008022 protein C-terminus binding
GO:0008283 cell proliferation
GO:0042802 identical protein binding
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0019903 protein phosphatase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0042060 wound healing
GO:0043235 receptor complex
GO:0043406 positive regulation of MAP kinase activity
GO:0045449 regulation of transcription
GO:0045765 regulation of angiogenesis
GO:0045785 positive regulation of cell adhesion
GO:0046777 protein autophosphorylation
GO:0046982 protein heterodimerization activity
GO:0048471 perinuclear region of cytoplasm
GO:0050679 positive regulation of epithelial cell proliferation

MCA1788A488

1808

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