EFTUD2 antibody | AB03/1B9

100% Secure


Mouse anti EFTUD2

Product Type
PrecisionAb Monoclonal
Clone
AB03/1B9
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00890
Datasheet
IP WB
SDS
100 µl loader

Mouse anti EFTUD2 antibody recognizes 116 kDa U5 small nuclear ribonucleoprotein component, also known as hSNU114.

EFTUD2 is a GTPase responsible for mRNA maturation and mutations. Binding of GTP/GDP to EFTUD2 plays an important role in spliceosome dynamics, and disruption of the EFTUD2 GTP-binding domain in yeast leads to unspliced mRNAs and lethality (Lei et al. 2017). The protein is reported to regulate the innate immune response through alternative splicing of MyD88, an important signaling adaptor in many TLR signaling pathways (Zhu et al. 2015). Specific variants in the EFTUD2 gene are associated with microcephaly (Rengasamy Venugopalan et al. 2017), and EFTUS2 haploinsufficiency is linked to a number of conditions including syndromic oesophageal atresia, a relatively common but life-threatening congenital malformation (Gordon et al. 2012).

Product Details

Target Species
Human
Western Blotting
Mouse anti EFTUD2 antibody detects a band of approximately 122 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-472 of human EFTUD2
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q15029
Entrez Gene
EFTUD2
GO Terms
GO:0005515 protein binding
GO:0003924 GTPase activity
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0005525 GTP binding
GO:0005737 cytoplasm
GO:0005681 spliceosomal complex
GO:0015030 Cajal body
GO:0016607 nuclear speck
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of EFTUD2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra