DNAJA2 antibody | OTI3A11

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Mouse anti DNAJA2

DNAJA2 antibody recognizes Dnaj homolog subfamily A, member 2, also known as HIRA interacting protein 4, cell cycle progression 3 protein, or renal carcinoma antigen NY-REN-14. This antibody has been extensively validated for western blotting using whole cell lysates.

DNAJA2 Antibody with Control Lysate

DNAJA2 antibody recognizes Dnaj homolog subfamily A, member 2, also known as HIRA interacting protein 4, cell cycle progression 3 protein, or renal carcinoma antigen NY-REN-14. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI3A11
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00274 WB 100 µl
VMA00274KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human DNAJA2 antibody recognizes the DnaJ homolog subfamily A member 2, also known as HIRA interacting protein 4, HIRA-interacting protein 4, cell cycle progression 3 protein, cell cycle progression restoration gene 3 protein, dnaJ homolog subfamily A member 2 and renal carcinoma antigen NY-REN-14.

Encoded by DNAJA2 gene, DnaJ homolog subfamily A member 2 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of DNAJA2 works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro (provided by RefSeq, Jul 2008).

Mouse anti Human DNAJA2 antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human DNAJA2 (NP_005871) produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
O60884 Related reagents
Entrez Gene
DNAJA2 Related reagents
GO Terms
GO:0051082 unfolded protein binding
GO:0006457 protein folding
GO:0016020 membrane
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0008284 positive regulation of cell proliferation
GO:0009408 response to heat
GO:0031072 heat shock protein binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of DNAJA2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti DNAJA2 detects a band of approximately 46 kDa in HEK293 cell lysates
Western Blotting
Anti DNAJA2 detects a band of approximately 46 kDa in HEK293 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB