DNAJA1 antibody | AB01/4A12

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Anti DNAJA1 Antibody, clone AB01/4A12 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti DNAJA1 Antibody, clone AB01/4A12 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti DNAJA1 Antibody, clone AB01/4A12 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti DNAJA1 Antibody, clone AB01/4A12 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with DNAJA1 antibody (1/1000, **VMA00803**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to DNAJA1 (molecular weight 46 kDa)

Anti DNAJA1 Antibody, clone AB01/4A12 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against DNAJA1 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti DNAJA1 antibody (**VMA00803**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 148 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.

Anti DNAJA1 Antibody, clone AB01/4A12 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of DNAJA1 from whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against DNAJA1 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Mouse anti DNAJA1

Product Type: PrecisionAb Monoclonal
Clone: AB01/4A12
Isotype: IgG2b
Format: Purified
Specificity: DNAJA1

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00803 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti DNAJA1 antibody recognizes DnaJ homolog subfamily A member 1, also known as DNAJ2, HDJ2, HSJ2, and HSPF4.

DNAJA1 is a member of the DNAJ family of proteins, the largest and most diverse family of co-chaperones that work with HCP70 and HSP90 (Ergülen et al. 2016). The DNAJ family has diverse involvement in cellular activities including protein translation, folding / unfolding / refolding, translocation and degradation (Parrales et al. 2016). DNAJA1 appears to play roles in autoimmune diseases, neurodegenerative diseases, and cancer (Ergülen et al. 2016). DNAJA1 also co-opted by and acts as a positive replicator for replication of the influenza A virus via its RNA polymerase activity (Cao et al. 2014).

Target Species: Human
Western Blotting: Mouse anti DNAJA1 antibody detects a band of approximately 46 kDa in HEK293 cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 1-397 of human DNAJA1
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P31689
Entrez Gene: DNAJA1
GO Terms: GO:0051082 unfolded protein binding; GO:0006457 protein folding; GO:0006986 response to unfolded protein; GO:0016020 membrane; GO:0046872 metal ion binding; GO:0005524 ATP binding; GO:0009408 response to heat; GO:0031072 heat shock protein binding; GO:0050750 low-density lipoprotein particle receptor binding; View More GO Terms