CTGF antibody | AB01/1E5

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Mouse anti CTGF

Product Type
PrecisionAb Monoclonal
Clone
AB01/1E5
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00685
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human CTGF antibody recognizes the connective tissue growth factor, also known as CCN2, IGF-binding protein 8, and IGFBP-8.

CTGF is a matricellular protein that can simultaneously bind to multiple ligands and modifies a variety of growth factors and cytokines. In this way, CTGF contributes to cell migration, proliferation and inflammation (Toda et al. 2018). CTGF, activated by pro-fibrotic TGF-beta (Song et al. 2017a), plays an essential role in fibrosis of multiple tissues, including skin, heart, liver, lung, and kidney (Makino et al. 2017). Fibrosis is a strong risk factor for several cancer types, including hepatocellular carcinoma (HCC) (Sakurai and Kudo 2013) and lung cancer (Park et al. 2001). Increased expression of CTGF in tumor cells is associated with worse prognosis, and knockdown of CTGF has resulted in greater therapeutic efficacy of anticancer drugs in hepatocellular carcinoma (Song et al. 2017b).

Product Details

Target Species
Human
Western Blotting
Mouse anti CTGF detects a band of approximately 36 kDa in MCF7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-349 of human CTGF
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P29279
Entrez Gene
CTGF
GO Terms
GO:0005886 plasma membrane
GO:0006260 DNA replication
GO:0001558 regulation of cell growth
GO:0005520 insulin-like growth factor binding
GO:0005578 proteinaceous extracellular matrix
GO:0008201 heparin binding
GO:0008544 epidermis development
GO:0009611 response to wounding
GO:0044255 cellular lipid metabolic process
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of CTGF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml