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CDK2 antibody | 1A6

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Anti CDK2 Antibody, clone 1A6 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti CDK2 Antibody, clone 1A6 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti CDK2 Antibody, clone 1A6 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti CDK2 Antibody, clone 1A6 (PrecisionAb Monoclonal Antibody) thumbnail image 4
Anti CDK2 Antibody, clone 1A6 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of CDK2 knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Mouse anti CDK2 antibody (VMA00126) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 37 second and (B) 30 second exposure.
Anti CDK2 Antibody, clone 1A6 (PrecisionAb Monoclonal Antibody) gallery image 2
Normalized signal intensity of CDK2 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.
Anti CDK2 Antibody, clone 1A6 (PrecisionAb Monoclonal Antibody) gallery image 3
Western blot analysis of whole cell lysates probed with Mouse anti Human CDK2 antibody (VMA00126) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 30 second exposure. Arrow points to CDK2 (molecular weight 34 kDa).
Anti CDK2 Antibody, clone 1A6 (PrecisionAb Monoclonal Antibody) gallery image 4
Immunoprecipitation of CDK2 from Jurkat whole cell lysate. The Input lane is 10% input; the CDK2-IP lane is the immunoprecipitation with the CDK2 antibody (2.5 µg, VMA00126); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 μg, MCA928). Western blot analysis was performed using CDK2 antibody (1/1,000, VMA00126) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to CDK2.

Mouse anti CDK2

Product Type
PrecisionAb Monoclonal
Clone
1A6
Isotype
IgG1
Format
Purified
Specificity
CDK2

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Product Code Applications Pack Size List Price Your Price Qty
VMA00126
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SDS Safety Datasheet SDS
IP WB 100 µl loader
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Mouse anti Human CDK2 antibody recognizes CDK2, also known as cdc2-related protein kinase, cell division protein kinase 2 and p33 protein kinase.

The protein encoded by the CDK2 gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of CDK2 have been reported (provided by RefSeq, Jul 2008).

Mouse anti Human CDK2 antibody detects a band of 34 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti CDK2 detects a band of approximately 34 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Purified recombinant fragment of human CDK2 expressed in E. coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P24941
Entrez Gene
CDK2
GO Terms
GO:0006260 DNA replication
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000085 G2 phase of mitotic cell cycle
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0000307 cyclin-dependent protein kinase holoenzyme complex
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004693 cyclin-dependent protein kinase activity
GO:0005524 ATP binding
GO:0006275 regulation of DNA replication
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0007067 mitosis
GO:0007089 traversing start control point of mitotic cell cycle
GO:0007265 Ras protein signal transduction
GO:0008284 positive regulation of cell proliferation
GO:0042802 identical protein binding
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0051301 cell division
GO:0051439 regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle

VMA00126

120615

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