CD71 (soluble) antibody | 13E4

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Mouse anti CD71 (soluble)

Product Type
PrecisionAb Monoclonal
Product Code Applications Pack Size List Price Quantity
100 µl loader

Mouse anti Human CD71 (soluble) antibody recognizes the human soluble transferrin receptor (sTfR) also known as sCD71 or p90. CD71 is a single pass type II membrane glycoprotein of ~90 kDa containing a single protease associated domain. sCD71 forms a homodimeric complex, a truncated form of the membrane bound transferrin receptor lacking the cytoplasmic and transmembrane regions of the molecule (Shih et al. 1990). It is responsible for the uptake of iron into the cell by binding to and internalizing iron-bound transferrin (Kogan et al. 2005). After the iron is released into the cell the apotransferrin is relocated to the cell surface, cleaved at Arg-100 and released into the circulation. In healthy adults almost all transferrin receptors exist in the soluble form (Shih et al. 1993). The level of soluble CD71 is used to detect erythropoietin misuse by athletes (Borrione et al. 2007). It is also used to diagnose iron deficiency anemia (Kamer et al. 2012).

Mouse anti Human soluble CD71 recognizes human both the soluble transferrin receptor and the complex between soluble transferrin receptor and transferrin. The antibody recognizes CD71 as a single band of ~90 kDa in multiple human cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Western Blotting
Anti CD71 (soluble) antibody detects a band of approximately 90 kDa in K562 cell lysates.
Product Form
Purified IgG - liquid
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Purified human soluble transferrin receptor
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0008233 peptidase activity
GO:0004998 transferrin receptor activity
GO:0005887 integral to plasma membrane
GO:0005768 endosome
GO:0005905 coated pit
GO:0006508 proteolysis
GO:0006879 cellular iron ion homeostasis
GO:0006897 endocytosis
GO:0033572 transferrin transport
GO:0042470 melanosome
GO:0044419 interspecies interaction between organisms
GO:0055085 transmembrane transport
PrecisionAb is a trademark of Bio-Rad Laboratories.
For research purposes only

Applications of CD71 (soluble) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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