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CD68 antibody | KP1

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Anti Human CD68 Antibody, clone KP1 thumbnail image 1 Anti Human CD68 Antibody, clone KP1 thumbnail image 2 Anti Human CD68 Antibody, clone KP1 thumbnail image 3 Anti Human CD68 Antibody, clone KP1 thumbnail image 4
Anti Human CD68 Antibody, clone KP1 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD14 (MCA1568F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Human CD14 (MCA1568F) and Alexa Fluor 647 conjugated Mouse anti Human CD68 (MCA5709A647). All experiments performed on Leucoperm (BUF09A) fixed and permeabilized human peripheral blood gated on single cell mononuclear cells. Data acquired on the ZE5 Cell Analyzer
Anti Human CD68 Antibody, clone KP1 gallery image 2
Immunoperoxidase staining of formalin fixed, paraffin embedded human tonsil stained with Mouse anti Human CD68 antibody, clone KP1 (MCA5709) following heat mediated antigen retrieval using citrate buffer (pH6.0). Staining visualized using Horseradish peroxidase conjugated Goat anti Mouse IgG.
Anti Human CD68 Antibody, clone KP1 gallery image 3
Immunoperoxidase staining of formalin fixed, paraffin embedded human breast carcinoma with Mouse anti Human CD68 antibody, clone KP1 (MCA5709).
Anti Human CD68 Antibody, clone KP1 gallery image 4
Published customer image:
Mouse anti human CD68 antibody, clone KP1 (MCA5709) used to identify macrophages in optic nerve tissue from the Northern Leopard Frog (Lithobates (Rana) pipiens) by immunofluorescence.
Image caption:
Immunostaining of macrophage subtypes.
(A—D) Confocal fluorescence micrographs of immunostained frozen sections of optic nerve 1 week after axotomy. (B) High magnification single slice of a macrophage with an irregular nucleus, clearly showing ED1-LI-stained lysosomes. (C) Double staining of peripherally-located macrophages with ED1 and Arg1. Only a few cells have predominantly ED1-LI with little Arg1-LI (asterisks). (D) Double staining of centrally-located macrophages with ED1 and Arg1. Only a few cells have predominantly ED1-LI (asterisks).

From: Blanco RE, Vega-Meléndez GS, De La Rosa-Reyes V, del Cueto C, Blagburn JM.
Application of CNTF or FGF-2 increases the number of M2-like macrophages after optic nerve injury in adult Rana pipiens.
PLoS ONE (2019) 14(5): e0209733.
doi: 10.1371/journal.pone.0209733
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Human CD68:FITC

Product Type
Monoclonal Antibody
Clone
KP1
Isotype
IgG1
Specificity
CD68

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Product Code Applications Pack Size List Price Your Price Qty
MCA5709F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * IF 0.1 mg loader
List Price Your Price
loader

Mouse anti human CD68 antibody, clone KP1 recognizes the human CD68 cell surface antigen, a ~110 kDa glycoprotein primarily expressed by macrophages and monocytes.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Subcellular fraction of human alveolar macrophages
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/10
Immunofluorescence
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock
Leucoperm BUF09 F 50 Tests
List Price Your Price
Description Leucoperm

Source Reference

  1. Pulford, K.A. et al. (1989) KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections.
    J Clin Pathol. 42 (4): 414-21.

References for CD68 antibody

  1. Masaki, T. et al. (2004) Activation of the extracellular-signal regulated protein kinase pathway in human glomerulopathies.
    J Am Soc Nephrol. 15 (7): 1835-43.
  2. Vroon, A. et al. (2007) Neuroinflammation in Parkinson's patients and MPTP-treated mice is not restricted to the nigrostriatal system: microgliosis and differential expression of interleukin-1 receptors in the olfactory bulb.
    Exp Gerontol. 42 (8): 762-71.
  3. Schwarz, F. et al. (2016) Human-specific derived alleles of CD33 and other genes protect against postreproductive cognitive decline.
    Proc Natl Acad Sci U S A. 113 (1): 74-9.
  4. Xiao, J. et al. (2016) Effects of ketanserin on experimental colitis in mice and macrophage function.
    Int J Mol Med. 37 (3): 659-68.
  5. Blanco, R.E. et al. (2019) Application of CNTF or FGF-2 increases the number of M2-like macrophages after optic nerve injury in adult Rana pipiens..
    PLoS One. 14 (5): e0209733.
  6. Hu, M. et al. (2022) Implications of Sm22α-Cre expression in keratinocytes and unanticipated inflammatory skin lesion in a model of atherosclerosis.
    Am J Physiol Heart Circ Physiol. 323 (3): H528-H534.

Immunofluorescence

Immunohistology - Paraffin

Synonyms
Macrosialin
RRID
AB_11152780
UniProt
P34810
Entrez Gene
CD68
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005624 membrane fraction
GO:0005765 lysosomal membrane
GO:0010008 endosome membrane

MCA5709F

1801

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