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CD68 antibody | KP1

Product Code Applications Pack Size List Price Your Price Qty
MCA5709F
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Mouse anti human CD68 antibody, clone KP1 recognizes the human CD68 cell surface antigen, a ~110 kDa glycoprotein primarily expressed by macrophages and monocytes.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Subcellular fraction of human alveolar macrophages
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/10
Immunofluorescence
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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List Price Your Price
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Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock
Leucoperm BUF09 F 50 Tests
List Price Your Price
Description Leucoperm

Source Reference

  1. Pulford, K.A. et al. (1989) KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections.
    J Clin Pathol. 42 (4): 414-21.

References for CD68 antibody

  1. Masaki, T. et al. (2004) Activation of the extracellular-signal regulated protein kinase pathway in human glomerulopathies.
    J Am Soc Nephrol. 15 (7): 1835-43.
  2. Vroon, A. et al. (2007) Neuroinflammation in Parkinson's patients and MPTP-treated mice is not restricted to the nigrostriatal system: microgliosis and differential expression of interleukin-1 receptors in the olfactory bulb.
    Exp Gerontol. 42 (8): 762-71.
  3. Schwarz, F. et al. (2016) Human-specific derived alleles of CD33 and other genes protect against postreproductive cognitive decline.
    Proc Natl Acad Sci U S A. 113 (1): 74-9.
  4. Xiao, J. et al. (2016) Effects of ketanserin on experimental colitis in mice and macrophage function.
    Int J Mol Med. 37 (3): 659-68.
  5. Blanco, R.E. et al. (2019) Application of CNTF or FGF-2 increases the number of M2-like macrophages after optic nerve injury in adult Rana pipiens..
    PLoS One. 14 (5): e0209733.
  6. Hu, M. et al. (2022) Implications of Sm22α-Cre expression in keratinocytes and unanticipated inflammatory skin lesion in a model of atherosclerosis.
    Am J Physiol Heart Circ Physiol. 323 (3): H528-H534.
  7. Robinson, H. et al. (2022) The effect of expressive writing on wound healing: Immunohistochemistry analysis of skin tissue two weeks after punch biopsy wounding.
    J Psychosom Res. 161: 110987.

Immunofluorescence

Immunohistology - Paraffin

Synonyms
Macrosialin
RRID
AB_11152780
UniProt
P34810
Entrez Gene
CD68
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005624 membrane fraction
GO:0005765 lysosomal membrane
GO:0010008 endosome membrane

MCA5709F

1801

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