CD61 antibody | Y2/51

Mouse anti Human CD61:RPE

Product Type
Monoclonal Antibody
Clone
Y2/51
Isotype
IgG1
Specificity
CD61

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MCA2588PE
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Mouse anti Human CD61 antibody, clone Y2/51 recognizes CD61, a ~105 kDa glycoprotein, also known as integrin beta 3 chain. This molecule associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51.

CD61 is a receptor for fibrinogen, fibronectin, vWF, vitronectin and thrombospondin.

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
5%Sucrose
Immunogen
PHA stimulated peripheral blood cells.
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
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References for CD61 antibody

  1. Phillips, D.R. et al. (1991) GPIIb-IIIa: the responsive integrin.
    Cell. 65 (3): 359-62.
  2. Hynes, R.O. (1992) Integrins: versatility, modulation, and signaling in cell adhesion.
    Cell. 69 (1): 11-25.
  3. Michelson, A. D. et al. (1995) A panel of platelet mAb for the study of haemostasis and thrombosis in baboons.
    Leucocyte Typing V. Oxford University Press p 1230-1.
  4. Gatter, K.C. et al. (1988) The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens.
    Histopathology. 13 (3): 257-67.
  5. Kamat, V. et al. (2015) Microfluidic assessment of functional culture-derived platelets in human thrombi under flow.
    Exp Hematol. 43 (10): 891-900.e4.
  6. Ogino, T. et al. (2014) Erythroid and megakaryocytic differentiation of K562 erythroleukemic cells by monochloramine.
    Free Radic Res. 48 (3): 292-302.
  7. Tehrani, S. et al. (2010) Atorvastatin has antithrombotic effects in patients with type 1 diabetes and dyslipidemia.
    Thromb Res. 126 (3): e225-31.
  8. Dircks, B.H. et al. (2012) Platelet-neutrophil aggregate formation in blood samples from dogs with systemic inflammatory disorders.
    Am J Vet Res. 73 (7): 939-45.
  9. View The Latest Product References
  10. Comazzi, S. et al. (2010) Acute megakaryoblastic leukemia in dogs: a report of three cases and review of the literature.
    J Am Anim Hosp Assoc. 46 (5): 327-35.
  11. Jackson, J.W. et al. (2016) Novel Antiplatelet Activity of Minocycline Involves Inhibition of MLK3-p38 Mitogen Activated Protein Kinase Axis.
    PLoS One. 11 (6): e0157115.
  12. Chen, C.P. et al. (2018) Oxidative stress reduces trophoblast FOXO1 and integrin β3 expression that inhibits cell motility.
    Free Radic Biol Med. Jun 07 [Epub ahead of print].

Synonyms
Integrin Beta 3 Chain
RRID
AB_2280594
UniProt
P05106
Entrez Gene
ITGB3
GO Terms
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002576 platelet degranulation
GO:0050839 cell adhesion molecule binding
GO:0004872 receptor activity
GO:0005161 platelet-derived growth factor receptor binding
GO:0008305 integrin complex
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0010595 positive regulation of endothelial cell migration
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010888 negative regulation of lipid storage
GO:0014909 smooth muscle cell migration
GO:0030168 platelet activation
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0031092 platelet alpha granule membrane
GO:0032147 activation of protein kinase activity
GO:0032369 negative regulation of lipid transport
GO:0035295 tube development
GO:0043184 vascular endothelial growth factor receptor 2 binding
GO:0044419 interspecies interaction between organisms
GO:0045124 regulation of bone resorption
GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050748 negative regulation of lipoprotein metabolic process
GO:0050900 leukocyte migration
GO:0060055 angiogenesis involved in wound healing
GO:0071062 alphav-beta3 integrin-vitronectin complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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