CD62P antibody | Psel.KO.2.5

ISO 9001 Quality Assurance Manufacturing |

Mouse anti Human CD62P:StarBright Blue 700

Product Type: Monoclonal Antibody
Clone: Psel.KO.2.5
Isotype: IgG1
Specificity: CD62P

Quick Links:

References (9)
Applications
Useful Reagents
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

24 Formats Available:

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA2418SBB700 Datasheet SDS SDS	F *	100 Tests/0.5ml		Log in
	Log in

Anti Human CD62P Antibody, clone Psel.KO.2.5 thumbnail image 1

Anti Human CD62P Antibody, clone Psel.KO.2.5 thumbnail image 2

Anti Human CD62P Antibody, clone Psel.KO.2.5 thumbnail image 3

Anti Human CD62P Antibody, clone Psel.KO.2.5 thumbnail image 4

Anti Human CD62P Antibody, clone Psel.KO.2.5 thumbnail image 5

Anti Human CD62P Antibody, clone Psel.KO.2.5 gallery image 1 — Figure A. FITC conjugated Mouse anti Human CD61 antibody, clone Y2/51 (**MCA2588F**) and Pacific Blue conjugated Mouse IgG1 isotype control (**MCA928PB**).
Figure B and C. FITC conjugated Mouse anti Human CD61 antibody, clone Y2/51 (**MCA2588F**) and Pacific Blue conjugated Mouse anti Human CD62P antibody, clone Psel.KO.2.5 (**MCA2418PB**). All experiments performed on Human blood gated on single platelets, in the presence of 10% human serum. Platelets in Figure C were thrombin activated.
Data acquired on the **ZE5 Cell analyser**.

Anti Human CD62P Antibody, clone Psel.KO.2.5 gallery image 2 — Figure A. FITC conjugated Mouse anti Human CD61 antibody, clone Y2/51 (**MCA2588F**) and Alexa Fluor^® 700 conjugated Mouse IgG1 isotype control (**MCA928A700**).
Figure B. FITC conjugated Mouse anti Human CD61 antibody, clone Y2/51 (**MCA2588F**) and Alexa Fluor^® 700 conjugated Mouse anti Human CD62P antibody, clone Psel.KO.2.5 (**MCA2418A700**). All experiments performed on thrombin activated Human platelets gated on platelets, in the presence of 10% human serum.
Data acquired on the **ZE5 Cell analyser**.

Anti Human CD62P Antibody, clone Psel.KO.2.5 gallery image 3 — Figure A. FITC conjugated Mouse anti Human CD61 antibody, clone Y2/51 (**MCA2588F**) and Alexa Fluor^® 647 conjugated Mouse IgG1 isotype control (**MCA928A647**).
Figure B. FITC conjugated Mouse anti Human CD61 antibody, clone Y2/51 (**MCA2588F**) and Alexa Fluor^® 647 conjugated Mouse anti Human CD62P antibody, clone Psel.KO.2.5 (**MCA2418A647**). All experiments performed on human blood gated on single platelets activated with thrombin, in the presence of 10% Human serum.
Data acquired on the **ZE5 Cell analyser**.

Anti Human CD62P Antibody, clone Psel.KO.2.5 gallery image 4 — Figure A. Alexa Flour^® 647 conjugated Mouse anti Human CD61 antibody, clone Y2/51 (**MCA2588A647**).
Figure B. Alexa Flour^® 647 conjugated Mouse anti Human CD61 antibody, clone Y2/51 (**MCA2588A647**) and StarBright Blue 700 conjugated Mouse anti Human CD62P antibody, clone Psel.KO.2.5 (**MCA2418SBB700**). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the **ZE5 Cell Analyzer**.

Anti Human CD62P Antibody, clone Psel.KO.2.5 gallery image 5 — Figure A. FITC conjugated Mouse anti Human CD61 antibody, clone Y2/51 (**MCA2588F**).
Figure B. FITC conjugated Mouse anti Human CD61 antibody, clone Y2/51 (**MCA2588F**) and StarBright Violet 610 conjugated Mouse anti Human CD62P antibody, clone Psel.KO.2.5 (**MCA2418SBV610**). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the **ZE5 Cell analyser**.

Filter by Application:

Mouse anti Human CD62P antibody, clone Psel.KO.2.5 recognizes the CD62P cell surface antigen, a ~140 kDa glycoprotein, also known as P-selectin.

CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Pig

Sheep; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG conjugated to StarBright Blue 700 - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen: CD62P transfected 300.19 cells.
Approx. Protein Concentrations: For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
Fusion Partners: Spleen cells from immunised CD62P knock-out mice (strain C57/B6) were fused with cells of the NS-1 myeloma cell line.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

StarBright Blue 700 473 703
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹		Neat

¹Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 5μl of the suggested working dilution to label 0.5x10⁶ cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸

Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra

Description	Product Code	Applications	Pack Size	Your Price
Human Seroblock	BUF070A	F	50 Test	Log in
List Price	Your Price
	Log in
Description	Human Seroblock
Human Seroblock	BUF070B	F	200 Test	Log in
List Price	Your Price
	Log in
Description	Human Seroblock

Source Reference

Massaguer, A. et al. (2000) Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).
Tissue Antigens. 56 (2): 117-28.

References for CD62P antibody

Massaguer, A. et al. (2003) Characterization of platelet and soluble-porcine P-selectin (CD62P).
Vet Immunol Immunopathol. 96 (3-4): 169-81.
Johnson, C.A. Jr. et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
Artif Organs. 35 (6): 602-13.
Krajewski, S. et al. (2012) Flow cytometry analysis of porcine platelets: optimized methods for best results.
Platelets. 23: 386-94.
Shankarraman, V. et al. (2014) Biocompatibility Assessment of the CentriMag-Novalung Adult ECMO Circuit in a Model of Acute Pulmonary Hypertension.
ASAIO J. 60 (4): 429-35.
Tunjungputri, R.N. et al. (2016) Invasive pneumococcal disease leads to activation and hyperreactivity of platelets.
Thromb Res. 144: 123-6.
Chan, C.H.H. et al. (2017) Shear Stress-Induced Total Blood Trauma in Multiple Species.
Artif Organs. 41 (10): 934-47.
Batchinsky, A.I. et al. (2023) Intravenous Autologous Bone-Marrow-derived Mesenchymal Stromal Cells Delay Acute Respiratory Distress Syndrome in Swine.
Am J Respir Crit Care Med. Oct 05 [Epub ahead of print].