CD56 antibody | 123C3
Mouse anti Human CD56 antibody, clone 123C3 (MCA2693GA) used to demonstrate CD56 expression on human NK cells by immunofluorescence.
Image caption:
Quantification of γH2AX foci in CD56+ NK cells (CD56, red; γH2AX, green) of patients following total body radiation. Representative images are shown. NK cells: At d1, d2 and d3 only very low numbers of CD56+ NK cells could be found on the slides because of high cytotoxicity. The surviving cells showed an increase of γH2AX foci with cumulative dosage.
From: Heylmann D, Ponath V, Kindler T, Kaina B.
Comparison of DNA repair and radiosensitivity of different blood cell populations.
Sci Rep. 2021 Jan 28;11(1):2478.
doi: 10.1038/s41598-021-81058-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD56 antibody, clone 123C3 (MCA2693GA) used to demonstrate CD56 expression on human NK cells by immunofluorescence.
Image caption:
Staining of γH2AX (green) and CD surface markers (red) of different blood cell types purified from buffy coat. (a) Representative pictures of unirradiated cells (control) and samples 1 and 24 h after 2 Gy IR. A maximum of γH2AX foci accumulate 1 h after exposure to 2 Gy. γH2AX foci disappear 24 h after radiation implicating effective repair of IR-induced DNA double-strand breaks. CD19+ B cells are hardly detectable (h.d.) on the slide 24 h after treatment. Furthermore, no CD34+ progenitor cells could be found on the slide 24 h post-irradiation (n.d. not detectable). Granulocytes, shown in combination with the CD15 marker, did not show any IR-induced formation of γ H2AX foci.
From: Heylmann D, Ponath V, Kindler T, Kaina B.
Comparison of DNA repair and radiosensitivity of different blood cell populations.
Sci Rep. 2021 Jan 28;11(1):2478.
doi: 10.1038/s41598-021-81058-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F C IF ResetMouse anti Human CD56:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 123C3
- Isotype
- IgG1
- Specificity
- CD56
Antibody Quality and Performance Guarantee
| Mouse anti Human CD56 antibody, clone 123C3 recognizes human neural cell adhesion molecule (NCAM), otherwise known as CD56. The CD56 molecule is a cell surface glycoprotein expressed on neuroendocrine cells, natural killer cells and a subset of T cells in the peripheral blood. Three main isoforms of CD56 exist. Neurons express the largest 180 kDa form, while hemopoietic cells express the 140 kDa isoform. Mouse anti Human CD56 antibody, clone 123C3 recognizes both the 140 kDa and the 180 kDa isoform of the CD56 protein. In neuronal tissues, CD56 mediates homophilic and heterophilic adhesion and is implicated in neural development. CD56 is also expressed on thyroid follicular epithelium and may play a role in autoimmune disease of the thyroid. CD56 is expressed in a range of tumors including tumors of the lung, neural derived malignancies and natural killer cell lymphomas. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Immunogen
- Membrane preparation of small lung carcinoma.
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the murine Sp2/0 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/5 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
|
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
References for CD56 antibody
-
Schol, D.J. et al. (1988) Monoclonal antibody 123C3, identifying small cell carcinoma phenotype in lung tumours, recognizes mainly, but not exclusively, endocrine and neuron-supporting normal tissues.
Int J Cancer Suppl. 2: 34-40. -
Mooi, W.J. et al. (1988) Monoclonal antibody 123C3 in lung tumour classification. Immunohistology of 358 resected lung tumours.
Mol Cell Probes. 2 (1): 31-7. -
Moolenaar, C.E. et al. (1990) Expression of neural cell adhesion molecule-related sialoglycoprotein in small cell lung cancer and neuroblastoma cell lines H69 and CHP-212.
Cancer Res. 50 (4): 1102-6. -
Aloysius, M.M. et al. (2010) Mucins and CD56 as markers of tumour invasion and prognosis in periampullary cancer.
Br J Surg. 97: 1269-78 -
Wanka, G. et al. (2020) LDOC1 as Negative Prognostic Marker for Vulvar Cancer Patients.
Int J Mol Sci. 21 (23) Dec 05 [Epub ahead of print]. -
Heylmann, D. et al. (2021) Comparison of DNA repair and radiosensitivity of different blood cell populations.
Sci Rep. 11 (1): 2478.
MCA2693A647
150670
163485
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