CD94 antibody | DX22

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Mouse anti Human CD94:StarBright UltraViolet 700

Product Type: Monoclonal Antibody
Clone: DX22
Isotype: IgG1
Specificity: CD94

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References (6)
Applications
Useful Reagents
Storage Information
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA2254SBUV700 Datasheet SDS SDS	F *	100 Tests/0.5ml		Log in
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Anti Human CD94 Antibody, clone DX22 thumbnail image 1

Anti Human CD94 Antibody, clone DX22 thumbnail image 2

Anti Human CD94 Antibody, clone DX22 thumbnail image 3

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Anti Human CD94 Antibody, clone DX22 thumbnail image 8

Anti Human CD94 Antibody, clone DX22 gallery image 1 — Staining of human peripheral blood lymphocytes with Mouse anti Human CD94 antibody, clone DX22 (**MCA2254**).

Anti Human CD94 Antibody, clone DX22 gallery image 2 — Staining of human peripheral blood lymphocytes with biotinylated Mouse anti Human CD94 antibody, clone DX22 (**MCA2254B**).

Anti Human CD94 Antibody, clone DX22 gallery image 3 — Staining of human peripheral blood lymphocytes with FITC conjugated Mouse anti Human CD94 antibody, clone DX22 (**MCA2254F**).

Anti Human CD94 Antibody, clone DX22 gallery image 4 — Staining of human peripheral blood lymphocytes with RPE conjugated Mouse anti Human CD94 antibody, clone DX22 (**MCA2254PE**).

Anti Human CD94 Antibody, clone DX22 gallery image 5 — Human whole blood was stained with Anti-Human CD56 Alexa Fluor^® 488 (**MCA2243A488**) only (Figure A) or with with Anti-Human CD56 Alexa Fluor^® 488 (**MCA2243A488**) and Anti-Human CD94 StarBright UltraViolet 540 (**MCA2254SBUV540**) (Figure B). Experiments were performed on isolated red-cell lysed blood, gated on live, single Lymphocytes. Data were acquired on a Cytek Aurora.

Anti Human CD94 Antibody, clone DX22 gallery image 6 — Human whole blood was stained with Anti-Human CD56 Alexa Fluor^® 488 (**MCA2693A488**) only (Figure A) or with with Anti-Human CD56 Alexa Fluor^® 488 (**MCA2693A488**) and Anti-Human CD94 StarBright UltraViolet 700 (**MCA2254SBUV700**) (Figure B). Experiments were performed on isolated red-cell lysed blood, gated on live, single Lymphocytes. Data were acquired on a Cytek Aurora.

Anti Human CD94 Antibody, clone DX22 gallery image 7 — Human whole blood was stained with Anti-Human CD56 Alexa Fluor^® 488 (**MCA2693A488**) only (Figure A) or with Anti-Human CD56 Alexa Fluor^® 488 (**MCA2693A488**) and Anti-Human CD94 StarBright UltraViolet 770 (**MCA2254SBUV770**) (Figure B). Experiments were performed on isolated red-cell lysed blood, gated on live, single Lymphocytes. Data were acquired on a Cytek Aurora.

Anti Human CD94 Antibody, clone DX22 gallery image 8 — Human whole blood was stained with Anti-Human CD56 Alexa Fluor^® 488 (**MCA2693A488**) only (Figure A) or with Anti-Human CD56 Alexa Fluor^® 488 (**MCA2693A488**) and Anti-Human CD94 StarBright Violet 535 (**MCA2254SBV535**) (Figure B). Experiments were performed on isolated red-cell lysed blood, gated on live, single Lymphocytes. Data were acquired on a Cytek Aurora.

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Mouse anti Human CD94 antibody, clone DX22 recognizes human Natural killer cells antigen CD94, also known as KLRD1, Killer cell lectin-like receptor, subfamily D, member 1, CD94, KP43 and NK cell receptor. CD94 is a 179 amino acid single pass type II transmembrane glycoprotein with a predicted molecular mass of ~20.5 kDa. There are 3 isoforms of CD94 derived by alternative splicing.

CD94 is expressed on natural killer (NK) cells and a subset of T lymphocytes.

CD94 is found to associate with NKG2 to form a heterodimer, involved in the inhibition of cell mediated cytotoxicity against cells bearing appropriate MHC class I allotypes.

Mouse anti Human CD94 antibody, clone DX22 is reported to inhibit the binding of CD94 to HLA-E (Braud et al. 1998) and HLA-G (Söderström. et al. 1997).

Target Species: Human
Product Form: Purified IgG conjugated to StarBright UltraViolet 700 - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen: Natural killer cell line.
Approx. Protein Concentrations: For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

StarBright UltraViolet 700 340 700
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹		Neat

¹Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 5μl of the suggested working dilution to label 0.5x10⁶ cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

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Source Reference

Phillips, J.H. et al. (1996) CD94 and a novel associated protein (94AP) form a NK cell receptor involved in the recognition of HLA-A, HLA-B, and HLA-C allotypes.
Immunity. 5 (2): 163-72.

References for CD94 antibody

Söderström K et al. (1997) CD94/NKG2 is the predominant inhibitory receptor involved in recognition of HLA-G by decidual and peripheral blood NK cells.
J Immunol. 159 (3): 1072-5.
Braud, V.M. et al. (1998) HLA-E binds to natural killer cell receptors CD94/NKG2A, B and C.
Nature. 391 (6669): 795-9.
Tomasec, P. et al. (2000) Surface expression of HLA-E, an inhibitor of natural killer cells, enhanced by human cytomegalovirus gpUL40.
Science. 287 (5455): 1031.
Hassold, N. et al. (2012) Enhancement of natural killer cell effector functions against selected lymphoma and leukemia cell lines by dasatinib.
Int J Cancer. 131 (6): E916-27.
Rongvaux, A. et al. (2014) Development and function of human innate immune cells in a humanized mouse model.
Nat Biotechnol. 32 (4): 364-72.

UniProt: Q13241
Entrez Gene: KLRD1
GO Terms: GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0004888 transmembrane receptor activity; GO:0005529 sugar binding; GO:0007166 cell surface receptor linked signaling pathway; GO:0050776 regulation of immune response