CD31 antibody | WM59

Mouse anti Human CD31:RPE

Product Type
Monoclonal Antibody
Clone
WM59
Isotype
IgG1
Specificity
CD31

Product Code Applications Pack Size List Price Your Price Qty
MCA1738PE
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SDS Safety Datasheet SDS
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MCA1738PET
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Mouse anti Human CD31 monoclonal antibody, clone WM59 recognizes the human CD31 antigen, a ~130 kDa single pass type I transmembrane glycoprotein bearing six C2 immunoglobulin domains. CD31 is expressed by all continuous endothelia including arteries, veins and non-sinusoidal capillaries, platelets,granulocytes and some lymphocytes. CD31 is not expressed by discontinuous endothelia such as hepatic sinusoids and splenic red pulp (Muller et al. 1989).CD31 is also known as PECAM-1.

The binding epitope for mouse anti human CD31, clone WM59 has been mapped to the Ig-like domain 2 (Fawcett et al. 1995).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Pack Size: 25 Tests
Reconstitute with 0.25ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Pack Size: 100 Tests
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 25 Tests
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/2 Pack Size: 100 Tests
Neat Pack Size: 25 Tests
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader
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Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

References for CD31 antibody

  1. Cabañas, C. et al. (1989) Characterization of a novel myeloid antigen regulated during differentiation of monocytic cells.
    Eur J Immunol. 19 (8): 1373-8.
  2. Fawcett, J. et al. (1995) Mapping the homotypic binding sites in CD31 and the role of CD31 adhesion in the formation of interendothelial cell contacts.
    J Cell Biol. 128:1229-41.
  3. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
    PLoS One. 7: e35577.
  4. Stockinger, H. et al. (1990) Molecular characterization and functional analysis of the leukocyte surface protein CD31.
    J Immunol. 145 (11): 3889-97.
  5. DeLisser, H.M. et al. (1994) Molecular and functional aspects of PECAM-1/CD31.
    Immunol Today. 15 (10): 490-5.
  6. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
    J Pharmacol Exp Ther. 321 (2): 656-62.
  7. Reedquist, K.A. et al. (2000) The small GTPase, Rap1, mediates CD31-induced integrin adhesion.
    J Cell Biol. 148: 1151-8.
  8. Vernon-Wilson, E.F. et al. (2007) CD31 delays phagocyte membrane repolarization to promote efficient binding of apoptotic cells.
    J Leukoc Biol. 82: 1278-88.
  9. View The Latest Product References
  10. Johnston, A. et al. (2005) The anti-inflammatory action of methotrexate is not mediated by lymphocyte apoptosis, but by the suppression of activation and adhesion molecules.
    Clin Immunol. 114: 154-63.
  11. Hilbe W et al. (2003) Immunohistochemical typing of non-small cell lung cancer on cryostat sections: correlation with clinical parameters and prognosis.
    J Clin Pathol. 56 (10): 736-41.
  12. Stein, A. et al. (2010) Local erythropoietin and endothelial progenitor cells improve regional cardiac function in acute myocardial infarction.
    BMC Cardiovasc Disord. Sep; 10:43.
  13. Woollard, K.J. et al. (2002) Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells.
    Clin Exp Immunol. 130: 256-62.
  14. Theberge, A.B. et al. (2015) Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesis.
    Anal Chem. 87 (6): 3239-46.
  15. Hilbe W et al. (2004) CD133 positive endothelial progenitor cells contribute to the tumour vasculature in non-small cell lung cancer.
    J Clin Pathol. 57 (9): 965-9.
  16. Yi, T. et al. (2015) Manufacture of Clinical-Grade Human Clonal Mesenchymal Stem Cell Products from Single Colony Forming Unit-Derived Colonies Based on the Subfractionation Culturing Method.
    Tissue Eng Part C Methods. 21 (12): 1251-62.
  17. Palakkan, A.A. et al. (2015) Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells.
    Biomed Rep. 3 (5): 626-636.
  18. Newey SE et al. (2014) The hematopoietic chemokine CXCL12 promotes integration of human endothelial colony forming cell-derived cells into immature vessel networks.
    Stem Cells Dev. 23 (22): 2730-43.
  19. Fabre-Mersseman V et al. (2011) CD4⁺ recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis.
    AIDS. 25 (9): 1153-62.
  20. Patten PE et al. (2008) CD38 expression in chronic lymphocytic leukemia is regulated by the tumor microenvironment.
    Blood. 111 (10): 5173-81.
  21. Katz SC et al. (2004) Liver sinusoidal endothelial cells are insufficient to activate T cells.
    J Immunol. 173 (1): 230-5.
  22. Pfisterer K et al. (2015) CD90(+) human dermal stromal cells are potent inducers of FoxP3(+) regulatory T cells.
    J Invest Dermatol. 135 (1): 130-41.
  23. Hale, S.J. et al. (2015) CXCR2 modulates bone marrow vascular repair and haematopoietic recovery post-transplant.
    Br J Haematol. 169 (4): 552-64.
  24. Muthana, M. et al. (2015) Directing cell therapy to anatomic target sites in vivo with magnetic resonance targeting.
    Nat Commun. 6: 8009.
  25. Schuster, C. et al. (2015) Development of Blood and Lymphatic Endothelial Cells in Embryonic and Fetal Human Skin.
    Am J Pathol. 185 (9): 2563-74.
  26. Somers, E. et al. (2016) Vascular Defects and Spinal Cord Hypoxia in Spinal Muscular Atrophy.
    Ann Neurol. 79 (2): 217-30.
  27. Soh, B.S. et al. (2016) Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells.
    Nat Commun. 7: 10774.
  28. GarikipatiV, N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
    PLoS One. 13 (2): e0192244.
  29. Duque, J.C. et al. (2019) Vascularization of the arteriovenous fistula wall and association with maturation outcomes.
    J Vasc Access. : 1129729819863584. [Epub ahead of print]
  30. Kim, J.S. et al. (2021) Randomization to Omega-3 Fatty Acid Supplementation and Endothelial Function in COPD: The COD-Fish Randomized Controlled Trial.
    Chronic Obstr Pulm Dis. 8(1): 41-53.
  31. Bye, A.P. et al. (2018) Immobilization of Nonactivated Unfixed Platelets for Real-Time Single-Cell Analysis.
    Methods Mol Biol. 1812: 1-11.
  32. Chai, S. et al. (2022) Identification of epithelial and mesenchymal circulating tumor cells in clonal lineage of an aggressive prostate cancer case.
    NPJ Precis Oncol. 6 (1): 41.

Flow Cytometry

Functional Assays

Immunohistology - Frozen

Synonyms
PECAM-1
RRID
AB_322883
UniProt
P16284
Entrez Gene
PECAM1
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