CD276 antibody | RM335

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Rabbit anti CD276

Product Type
Monoclonal Antibody
Clone
RM335
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6348
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti CD276 antibody recognizes CD276 antigen, also known as B7-H3.

CD276 is a type I transmembrane protein which acts as a co-stimulatory/co-inhibitory molecule in the immune system to induce cellular immunity (Zhang and Hao 2019). CD276 functions as an immune checkpoint protein induced on the surface of antigen-presenting cells and is important for inhibition of T cell function. This protein is overexpressed in a range of solid cancers and tumor-infiltrating blood vessels and frequently correlates with negative prognosis and poor clinical outcome (Picarda et al. 2017). Anti-CD276 drug conjugates have been suggested as promising anti-cancer therapies, due to the overexpression of CD276 on tumors making it possible to distinguish between physiological and pathological processes (Seaman et al. 2018).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the C-terminus of human CD276

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q5ZPR3
Entrez Gene
CD276
GO Terms
GO:0005102 receptor binding
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0008283 cell proliferation
GO:0009897 external side of plasma membrane
GO:0042102 positive regulation of T cell proliferation
GO:0042110 T cell activation
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0050776 regulation of immune response
Regulatory
For research purposes only

Applications of CD276 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/500 1/1000
Western Blotting 1/5000 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Fluorescent Spectraviewer

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  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra