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CD24 antibody | SN3

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Anti Human CD24 Antibody, clone SN3 thumbnail image 1 Anti Human CD24 Antibody, clone SN3 thumbnail image 2 Anti Human CD24 Antibody, clone SN3 thumbnail image 3
Anti Human CD24 Antibody, clone SN3 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD66b (MCA216F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Human CD66b (MCA216F) and RPE conjugated Mouse anti Human CD24 (MCA1379PE). All experiments performed on red cell lysed human blood gated on myeloid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD24 Antibody, clone SN3 gallery image 2
Figure A. APC conjugated Mouse anti Human CD45 (MCA87APC) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. APC conjugated Mouse anti Human CD45 (MCA87APC) and FITC conjugated Mouse anti Human CD24 (MCA1379F). All experiments performed on red cell lysed human blood gated on live myeloid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD24 Antibody, clone SN3 gallery image 3
Figure A. FITC conjugated Mouse anti Human CD66b (MCA216F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Human CD66b (MCA216F) and Alexa Fluor 647 conjugated Mouse anti Human CD24 (MCA1379A647). All experiments performed on human blood gated on live single granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Human CD24:RPE

Product Type
Monoclonal Antibody
Clone
SN3
Isotype
IgG1
Specificity
CD24

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Product Code Applications Pack Size List Price Your Price Qty
MCA1379PE
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F 100 Tests loader
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Mouse anti Human CD24 antibody, clone SN3 recognizes the human CD24 cell surface antigen, a heavily glycosylated molecule of ~35-45 kDa. CD24 is expressed by granulocytes and B lymphocytes, and by some activated T cells and T cell malignancies.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chimpanzee
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Glycoproteins purified from human NALM-1 cell line
Fusion Partners
Spleen cells from immunized BALB/c mice were fuzed with cells of a mouse myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader
List Price Your Price
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Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD24 antibody

  1. Barcos, M. et al. (1986) Follicular mantle zone cell subpopulations detected by monoclonal antibody SN3.
    Hematol Oncol. 4 (4): 251-9.
  2. Fukukawa, T. et al. (1986) New monoclonal antibodies SN3, SN3a, and SN3b directed to sialic acid of glycoprotein on human non-T leukemia cells.
    Exp Hematol. 14 (9): 850-5.
  3. Sallustio, F. et al. (2010) TLR2 plays a role in the activation of human resident renal stem/progenitor cells.
    FASEB J. 24 (2): 514-25.
  4. Ohyama, M. et al. (2006) Characterization and isolation of stem cell-enriched human hair follicle bulge cells.
    J Clin Invest. 116:249-60.
  5. Kanderová, V. et al. (2010) Aberrantly expressed CEACAM6 is involved in the signaling leading to apoptosis of acute lymphoblastic leukemia cells.
    Exp Hematol. 38: 653-660.e1.
  6. Kap, Y.S. et al. (2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
    J Histochem Cytochem. 57 (12): 1159-67.
  7. Franz, C. et al. (2016) Extracellular vesicles in human follicular fluid do not promote coagulation.
    Reprod Biomed Online. Aug 22. pii: S1472-6483(16)30453-9. [Epub ahead of print]

Flow Cytometry

RRID
AB_321527
UniProt
P25063
Entrez Gene
CD24
GO Terms
GO:0005886 plasma membrane
GO:0001666 response to hypoxia
GO:0001775 cell activation
GO:0001959 regulation of cytokine-mediated signaling pathway
GO:0002237 response to molecule of bacterial origin
GO:0002768 immune response-regulating cell surface receptor signaling pathway
GO:0004871 signal transducer activity
GO:0016055 Wnt receptor signaling pathway
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0007411 axon guidance
GO:0008629 induction of apoptosis by intracellular signals
GO:0009986 cell surface
GO:0031225 anchored to membrane
GO:0016337 cell-cell adhesion
GO:0016477 cell migration
GO:0019901 protein kinase binding
GO:0030296 protein tyrosine kinase activator activity
GO:0030856 regulation of epithelial cell differentiation
GO:0031295 T cell costimulation
GO:0032597 B cell receptor transport into membrane raft
GO:0032600 chemokine receptor transport out of membrane raft
GO:0032913 negative regulation of transforming growth factor-beta3 production
GO:0042104 positive regulation of activated T cell proliferation
GO:0042632 cholesterol homeostasis
GO:0043406 positive regulation of MAP kinase activity
GO:0043408 regulation of MAPKKK cascade
GO:0043627 response to estrogen stimulus
GO:0045121 membrane raft
GO:0045730 respiratory burst

MCA1379PE

157765 INN0711R

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