CD20 antibody | 10F381
0.5 μg of Human Anti-CD20 Antibody, MCA6091 (rituximab biosimilar, research grade) and rituximab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.
Size exclusion chromatography (SEC) of Human Anti-CD20 Antibody, MCA6091 (rituximab biosimilar, research grade) and rituximab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl; flow rate: 200 μl/min; inj. volume [μl]: 2; temp.: 25 °C; detection: 280 nm; sample: mAb (0.5-5 mg/ml).
A microtiter plate was coated overnight with Human Anti-Rituximab Antibody (clone AbD18423, HCA186) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of rituximab (therapeutic reference product) or Human Anti-CD20 Antibody, MCA6091 (rituximab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Rituximab Antibody (clone AbD18423_hIgG1, HCA201) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®.
Human anti CD20 (Rituximab Biosimilar)
Anti-CD20 Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug rituximab (Rituxan) for research use. It can be used in bioanalytical PK and ADA assays and for studying biological pathways affected by the drug.
- Product Type
- Monoclonal Antibody
- Clone
- 10F381
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA6091 | E | 0.2 mg |
|
Human Anti-CD20 antibody, clone 10F381 is a research grade biosimilar of the monoclonal antibody drug rituximab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic rituximab antibody clone 10F381.
This antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-rituximab antibodies, for example HCA186 and HCA201. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-rituximab antibody in IgG1 format as a reference standard, for example HCA201.
Rituximab is a chimeric monoclonal antibody with murine variable region and human constant domains. It targets CD20, a protein found on the surface of B cells. Binding of the drug to its target triggers cell death through a number of mechanisms, including apoptosis, complement activation and cell-mediated cytotoxicity. Rituximab has been approved for the treatment of B cell malignancies, transplant rejection and autoimmune disorders.
This product is NOT FOR THERAPEUTIC USE.
View a summary of supporting anti-rituximab antibody products.
This antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-rituximab antibodies, for example HCA186 and HCA201. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-rituximab antibody in IgG1 format as a reference standard, for example HCA201.
Rituximab is a chimeric monoclonal antibody with murine variable region and human constant domains. It targets CD20, a protein found on the surface of B cells. Binding of the drug to its target triggers cell death through a number of mechanisms, including apoptosis, complement activation and cell-mediated cytotoxicity. Rituximab has been approved for the treatment of B cell malignancies, transplant rejection and autoimmune disorders.
This product is NOT FOR THERAPEUTIC USE.
View a summary of supporting anti-rituximab antibody products.
Product Details
- Target Species
- Human
- Product Form
- Human IgG1 (kappa) antibody - liquid
- Preparation
- Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Proclin 300
- Immunogen
- Human lymphoblastoid cell line SB
- Purity
- >98% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Licensed Use
- For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
- Regulatory
- For research purposes only
Applications of CD20 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Rituximab | HCA061 | E | 0.1 mg |
|
|
| Human anti Rituximab:HRP | HCA061P | E | 0.1 mg |
|
|
| Human anti Rituximab | HCA062 | E | 0.1 mg |
|
|
| Human anti Rituximab:HRP | HCA062P | E | 0.1 mg |
|
|
| Human anti Rituximab | HCA186 | E | 0.1 mg |
|
|
| Human anti Rituximab | HCA201 | E | 0.1 mg |
|
|
| LYNX Rapid HRP Antibody Conjugation Kit | LNK001P | CJ | 1 Conjugation For 400µg Antibody | ||
| Rat anti Rituximab | MCA2260 | E F | 0.2 mg |
|
|
| Rat anti Rituximab:FITC | MCA2260F | F | 0.1 mg |
|
|
| Rat anti Rituximab:HRP | MCA2260P | E | 0.1 mg |
|
