CD20 antibody | 10F381

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Human anti CD20 (Rituximab Biosimilar)

Anti-CD20 Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug rituximab (Rituxan) for research use. It can be used in bioanalytical PK and ADA assays and for studying biological pathways affected by the drug.

Product Type
Monoclonal Antibody
Clone
10F381
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6091
Datasheet
E
MSDS
0.2 mg loader

Human Anti-CD20 antibody, clone 10F381 is a research grade biosimilar of the monoclonal antibody drug rituximab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic rituximab antibody clone 10F381.

This antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-rituximab antibodies, for example HCA186 and HCA201. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-rituximab antibody in IgG1 format as a reference standard, for example HCA201.

Rituximab is a chimeric monoclonal antibody with murine variable region and human constant domains. It targets CD20, a protein found on the surface of B cells. Binding of the drug to its target triggers cell death through a number of mechanisms, including apoptosis, complement activation and cell-mediated cytotoxicity. Rituximab has been approved for the treatment of B cell malignancies, transplant rejection and autoimmune disorders.

This product is NOT FOR THERAPEUTIC USE.

View a summary of supporting anti-rituximab antibody products.

Product Details

Target Species
Human
Product Form
Human IgG1 (kappa) antibody - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Proclin 300
Immunogen
Human lymphoblastoid cell line SB
Purity
>98% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Licensed Use
For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
Regulatory
For research purposes only

Applications of CD20 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Rituximab HCA061 E 0.1 mg loader
Human anti Rituximab:HRP HCA061P E 0.1 mg loader
Human anti Rituximab HCA062 E 0.1 mg loader
Human anti Rituximab:HRP HCA062P E 0.1 mg loader
Human anti Rituximab HCA186 E 0.1 mg loader
Human anti Rituximab HCA201 E 0.1 mg loader
LYNX Rapid HRP Antibody Conjugation Kit LNK001P CJ 1 Conjugation For 400µg Antibody
Rat anti Rituximab MCA2260 E F 0.2 mg loader
Rat anti Rituximab:FITC MCA2260F F 0.1 mg loader
Rat anti Rituximab:HRP MCA2260P E 0.1 mg loader

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