CD19 antibody | LT19

Mouse anti Human CD19:StarBright UltraViolet 400

Product Type
Monoclonal Antibody
Clone
LT19
Isotype
IgG1
Specificity
CD19

Product Code Applications Pack Size List Price Your Price Qty
MCA1940SBUV400
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Mouse anti Human CD19 antibody, clone LT19 recognizes human CD19 also known as T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4. CD19 is a ~95 kDa type I single pass transmembrane glycoprotein expressed on follicular dendritic cells and B-cells during maturation but is lost on development into plasma cells (de Rie et al. 1989).

CD19 is the broadest lineage specific marker for B cells and functions as a B-cell co-receptor in conjunction with CD21 (Bradbury et al. 1992), CD9, CD81 and CD82 (Horváth et al. 1998). CD19 is implicated in the down-regulation of B cell growth and proliferation (Pezzutto et al. 1987).

Target Species
Human
Product Form
Purified IgG conjugated to StarBright UltraViolet 400 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright UltraViolet 400 347 394
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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References for CD19 antibody

  1. Hughes, G.J. et al. (2007) Virus immunocapture provides evidence of CD8 lymphocyte-derived HIV-1 in vivo.
    AIDS. 21: 1507-13.
  2. Allen, J.S. et al. (2009) Plasmacytoid dendritic cells are proportionally expanded at diagnosis of type 1 diabetes and enhance islet autoantigen presentation to T-cells through immune complex capture.
    Diabetes. 58: 138-45.
  3. McIntosh, K. et al. (2006) The immunogenicity of human adipose-derived cells: temporal changes in vitro.
    Stem Cells. 24: 1246-53.
  4. Sengstake, S. et al. (2006) CD21 and CD62L shedding are both inducible via P2X7Rs.
    Int Immunol. 18 (7): 1171-8.
  5. Villarroel Dorrego, M. et al. (2006) Transfection of CD40 in a human oral squamous cell carcinoma keratinocyte line upregulates immune potency and costimulatory molecules.
    Br J Dermatol. 154: 231-8.
  6. Franz, B. et al. (2011) Ex vivo characterization and isolation of rare memory B cells with antigen tetramers.
    Blood. 118: 348-57.
  7. Lacal, P.M. et al. (2013) Glucocorticoid-induced tumor necrosis factor receptor family-related ligand triggering upregulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 and promotes leukocyte adhesion.
    J Pharmacol Exp Ther. 347: 164-72.
  8. Franz, B. et al. (2011) Ex vivo characterization and isolation of rare memory B cells with antigen tetramers.
    Blood. 118: 348-57.
  9. View The Latest Product References
  10. Girbl, T. et al. (2013) CD40-mediated activation of chronic lymphocytic leukemia cells promotes their CD44-dependent adhesion to hyaluronan and restricts CCL21-induced motility.
    Cancer Res. 73: 561-70.
  11. Hertzberg, L. et al. (2010) Down syndrome acute lymphoblastic leukemia, a highly heterogeneous disease in which aberrant expression of CRLF2 is associated with mutated JAK2: a report from the International BFM Study Group.
    Blood. 115: 1006-17.
  12. Kakko, T. et al. (2011) Inflammatory effects of blood leukocytes: association with vascular function in neuropeptide Y proline 7-genotyped type 2 diabetes patients.
    Diab Vasc Dis Res. 8: 221-8.
  13. Dorvignit, D. et al. (2012) Expression and biological characterization of an anti-CD20 biosimilar candidate antibody: a case study.
    MAbs. 4 (4): 488-96.
  14. Karlsen, M. et al. (2015) TLR-7 and -9 Stimulation of Peripheral Blood B Cells Indicate Altered TLR Signalling in Primary Sjögren's Syndrome Patients by Increased Secretion of Cytokines.
    Scand J Immunol. 82 (6): 523-31.
  15. Clark, L.E. et al. (2018) Vaccine-elicited receptor-binding site antibodies neutralize two New World hemorrhagic fever arenaviruses.
    Nat Commun. 9 (1): 1884.
  16. Gu, Y. et al. (2019) Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01.
    Nat Commun. 10 (1): 893.
  17. Yang, C. et al. (2013) B cells promote tumor progression via STAT3 regulated-angiogenesis.
    PLoS One. 8 (5): e64159.

Flow Cytometry

UniProt
P15391
Entrez Gene
CD19
GO Terms
GO:0005515 protein binding
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0006968 cellular defense response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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