CD171 antibody | OTI2G9

Mouse anti CD171

Product Type
PrecisionAb Monoclonal
Clone
OTI2G9
Isotype
IgG1
Format
Purified
Specificity
CD171

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VMA00265
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Mouse anti Human CD171 antibody, clone OTI2G9 recognizes the CD171 antigen, also known as neural cell adhesion molecule L1, antigen identified by monoclonal antibody R1 and neural cell adhesion molecule L1.

Encoded by L1CAM gene, CD171 is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of L1CAM results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons (provided by RefSeq, May 2013).

Mouse anti Human CD171 antibody, clone OTI2G9 detects a band of 220-240 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti CD171 detects a band of approximately 220-240 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human CD171 (NP_000416) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P32004
Entrez Gene
L1CAM
GO Terms
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0007411 axon guidance
GO:0008219 cell death
GO:0050900 leukocyte migration
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