CD171 antibody | OTI2G9

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Anti CD171 Antibody, clone OTI2G9 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti CD171 Antibody, clone OTI2G9 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti CD171 Antibody, clone OTI2G9 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human CD171 antibody, clone OTI2G9 (**VMA00265**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 19 second exposure. Arrow points to CD171 (molecular weight 220-240 kDa).

Anti CD171 Antibody, clone OTI2G9 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of CD171 from HeLa whole cell lysate. The Input lane is 10% input; the CD171-IP lane is the immunoprecipitation with the CD171 antibody (2.5 µg, **VMA00265**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using CD171 antibody (1/1,000, **VMA00265**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to CD171.

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WB IP

Mouse anti CD171

Product Type: PrecisionAb Monoclonal
Clone: OTI2G9
Isotype: IgG1
Format: Purified
Specificity: CD171

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00265 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human CD171 antibody, clone OTI2G9 recognizes the CD171 antigen, also known as neural cell adhesion molecule L1, antigen identified by monoclonal antibody R1 and neural cell adhesion molecule L1.

Encoded by L1CAM gene, CD171 is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of L1CAM results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons (provided by RefSeq, May 2013).

Mouse anti Human CD171 antibody, clone OTI2G9 detects a band of 220-240 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti CD171 detects a band of approximately 220-240 kDa in HeLa cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human CD171 (NP_000416) produced in HEK293T cells
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P32004
Entrez Gene: L1CAM
GO Terms: GO:0007596 blood coagulation; GO:0016021 integral to membrane; GO:0007411 axon guidance; GO:0008219 cell death; GO:0050900 leukocyte migration