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Caspase-3 antibody | CD03/3D6

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Anti Caspase-3 Antibody, clone CD03/3D6 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Caspase-3 Antibody, clone CD03/3D6 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti Caspase-3 Antibody, clone CD03/3D6 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti Caspase-3 Antibody, clone CD03/3D6 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with caspase-3 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemicDoc MP with 51 second exposure. Arrow points to caspase-3 (molecular weight 32 kDa).
Anti Caspase-3 Antibody, clone CD03/3D6 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of Caspase-3 knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Mouse anti Caspase-3 antibody (VMA00412) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 75 second and (B) 30 second exposure.
Anti Caspase-3 Antibody, clone CD03/3D6 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of Caspase-3 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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WB

Mouse anti Caspase-3

Product Type
PrecisionAb Monoclonal
Clone
CD03/3D6
Isotype
IgG2b
Format
Purified
Specificity
Caspase-3

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Product Code Applications Pack Size List Price Your Price Qty
VMA00412
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human caspase-3 antibody, clone CD3/3D6 recognizes caspase-3, also known as CASP-3, CPP-32, PARP cleavage protease, SREBP cleavage activity 1 and protein Yama (protein of death).

Encoded by the CASP3 gene, caspase-3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in apoptosis or programmed cell death. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase-3 cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of CASP3 results in two transcript variants that encode the same protein (provided by RefSeq, Jul 2008).

Mouse anti Human caspase-3 antibody, clone CD3/3D6 detects a band of 32 kDa corresponding to the full-length active protein. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti caspase-3 detects a band of approximately 32 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E.coli-derived recombinant protein corresponding to aa 27-175 of human caspase-3
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P42574
Entrez Gene
CASP3
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0006508 proteolysis
GO:0008624 induction of apoptosis by extracellular signals
GO:0008629 induction of apoptosis by intracellular signals
GO:0008635 activation of caspase activity by cytochrome c
GO:0030264 nuclear fragmentation involved in apoptotic nuclear change
GO:0034612 response to tumor necrosis factor
GO:0043066 negative regulation of apoptosis
GO:0048011 nerve growth factor receptor signaling pathway

VMA00412

160303

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