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Casein Kinase 1 Epsilon antibody | OTI5C9

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siRNA Validated
Anti Casein Kinase 1 Epsilon Antibody, clone OTI5C9 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Casein Kinase 1 Epsilon Antibody, clone OTI5C9 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti Casein Kinase 1 Epsilon Antibody, clone OTI5C9 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti Casein Kinase 1 Epsilon Antibody, clone OTI5C9 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with casein kinase 1 epsilon antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 30 second exposure. Arrow points to casein kinase 1 epsilon (molecular weight 43 kDa)
Anti Casein Kinase 1 Epsilon Antibody, clone OTI5C9 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against Casein Kinase 1 Epsilon (1 & 2) or a scrambled siRNA (-) probed with Mouse anti Casein Kinase 1 Epsilon antibody (VMA00258) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 27 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.
Anti Casein Kinase 1 Epsilon Antibody, clone OTI5C9 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of Casein Kinase 1 Epsilon from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against Casein Kinase 1 Epsilon (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

Mouse anti Casein Kinase 1 Epsilon

Product Type
PrecisionAb Monoclonal
Clone
OTI5C9
Isotype
IgG2a
Format
Purified
Specificity
Casein Kinase 1 Epsilon

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Product Code Applications Pack Size List Price Your Price Qty
VMA00258
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human casein kinase 1 epsilon antibody recognizes the casein kinase I isoform epsilon, also known as CKI-epsilon, CKIe and casein kinase I isoform epsilon.

Encoded by the CSNK1E gene, casein kinase 1 epsilon is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for CSNK1E (provided by RefSeq, Jul 2008).

Mouse anti Human casein kinase I isoform epsilon antibody detects a band of 43 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti casein kinase 1 epsilon detects a band of approximately 43 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 153-416 of human casein kinase 1 epsilon (NP_689407) produced in E. coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P49674
Entrez Gene
CSNK1E
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0005515 protein binding
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0007165 signal transduction
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006281 DNA repair
GO:0006468 protein phosphorylation
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process

VMA00258

151112

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