C9orf72 antibody | B01-5F2
KO Validated
siRNA Validated
Mouse anti Human C9orf72 antibody, clone B01-5F2 (VMA00065) used to evaluate C9orf72 expression in normal and c9orf72 knockout human cell lysates by western blotting.
Image caption:
C9orf72 negatively regulates CARM1 protein level. (A,B) After glucose starvation for 0, 6, and 16 h, wild-type and C9KO MEFs were lysed, and CARM1 expression was determined by immunoblotting. n = 3. (C) Flag-tagged human C9orf72 was expressed in HEK293 cells, which were grown with or without glucose starvation for 6 h. The cells were then analyzed for CARM1 expression by immunoblotting. (D) The rescue of C9orf72 levels by stably expressing human C9orf72 in C9KO MEFs reversed the increase in CARM1 protein level under 6-h glucose starvation back to the wild-type level. (E,F) Cerebral cortex lysates from 2-mo-old wild-type and C9KO mice with or without 24-h fasting conditions were analyzed for CARM levels by immunoblotting. n = 3. (GS) Glucose starvation. Data are presented as mean ± SEM. (*) P <0.05; (**) P <0.01.
From: Liu Y, Wang T, Ji YJ, Johnson K, Liu H, Johnson K, Bailey S, Suk Y, Lu YN, Liu M, Wang J.
A C9orf72-CARM1 axis regulates lipid metabolism under glucose starvation-induced nutrient stress.
Genes Dev. 2018 Nov 1;32(21-22):1380-97.
doi: 10.1101/gad.315564.118.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human C9orf72 antibody, clone B01-5F2 (VMA00065) used to evaluate C9orf72 expression in mouse embryonic fibroblasts by immunofluorescence.
Image caption:
C9orf72 interacts with CARM1 and is required for its lysosomal degradation.
MEFs were fed or starved for 6 h with or without 100 nM bafilomycin A1 treatment. Lysosomal localization of endogenous C9orf72 and CARM1 was determined by immunostaining.
From: Liu Y, Wang T, Ji YJ, Johnson K, Liu H, Johnson K, Bailey S, Suk Y, Lu YN, Liu M, Wang J.
A C9orf72-CARM1 axis regulates lipid metabolism under glucose starvation-induced nutrient stress.
Genes Dev. 2018 Nov 1;32(21-22):1380-97.
doi: 10.1101/gad.315564.118.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB IF ResetMouse anti C9orf72
- Product Type
- PrecisionAb Monoclonal
- Clone
- B01-5F2
- Isotype
- IgG1
- Format
- Purified
- Specificity
- C9orf72
Antibody Quality and 
Unrivaled 
| Mouse anti Human C9orf72 antibody, clone B01-5F2 recognizes the protein C9orf72 Expansion of a hexanucleotide repeat in non-coding sequence between alternate 5' exons in transcripts from C9orf72 is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Multiple transcript variants encoding different isoforms have been found for C9orf72. (provided by RefSeq, Dec 2011). Mouse anti Human C9orf72 antibody detects a band of 54 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
|
- Target Species
- Human
- Western Blotting
- Anti C9orf72 detects a band of approximately 54 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified from serum free cell culture supernatant by Protein G column.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Immunogen
- Synthetic peptide of human C9orf72
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20&dec;C, avoiding repeated freeze thaw cycles
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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References for C9orf72 antibody
-
Liu, Y. et al. (2018) A C9orf72-CARM1 axis regulates lipid metabolism under glucose starvation-induced nutrient stress.
Genes Dev. 32 (21-22): 1380-97.
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