C5 antibody | h5G1.1
1 μg of Human Anti-C5 Antibody, MCA6145 (eculizumab biosimilar, research grade) was denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie Blue staining. The fully assembled antibody (H2L2), heavy (H) and light chains (L) are indicated. The molecular weight (kDa) of the marker proteins is displayed on the left hand side of the image.
Size exclusion (SE) chromatography of Human Anti-C5 Antibody, MCA6145 (eculizumab biosimilar, research grade) was performed on an analytical HPLC instrument. The larger peak corresponds to monomeric, fully assembled antibody (H2L2), the smaller peak contains oligomers. Column: MAbPac SEC-1.5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, 200mM arginine, 300mM NaCl, pH 6.8, flow rate: 200 μl/min; inj. volume: 3 μl; temp.: 25 °C; detection: 280 nm; sample: 1 mg/ml.
A microtiter plate was coated overnight with Human Anti-Eculizumab Antibody (clone AbD32311, HCA312) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% fetal calf serum was added spiked with increasing concentrations of Human Anti-C5 Antibody, MCA6145 (eculizumab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Eculizumab Antibody (AbD32107_hIgG1, HCA313) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK001P).
Human anti C5 (Eculizumab Biosimilar)
- Product Type
- Monoclonal Antibody
- Clone
- h5G1.1
- Isotype
- IgG2/4
- Specificity
- C5
Antibody Quality and Performance Guarantee
| Human Anti-C5 alpha antibody, clone h5G1.1, is a research grade biosimilar of the monoclonal antibody drug eculizumab. It is a recombinant human IgG2/4 kappa antibody with variable regions from the therapeutic eculizumab antibody clone h5G1.1. This product is supplied in a research grade format, i.e. in PBS buffer with preservative, it is not manufactured or formulated in the same way as the therapeutic reference product. A preservative free version is available on request. Human Anti-C5 Antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-eculizumab antibodies for example HCA312 and HCA313. Eculizumab (Soliris) is a humanized IgG2/4 kappa antibody. The heavy chains are comprised of human IgG2 sequences in CH1, the hinge and the adjacent portion CH2, and human IgG4 sequences in the remaining part of CH2 and CH3. The drug is used to treat paroxysmal nocturnal hemoglobinuria (PNH), a rare acquired life-threatening disease of the blood characterized by destruction of red blood cells by the complement system. It also has approval as an orphan drug for treatment of atypical hemolytic uremic syndrome (aHUS). Eculizumab specifically binds to the terminal complement component 5 (C5) and inhibits the cleavage of C5 to C5a and C5b by the C5 convertase, which prevents the generation of the terminal complement complex C5b-9. Both C5a and C5b-9 cause the terminal complement-mediated events that are characteristic of PNH and aHUS. A 1:1 molar ratio of eculizumab to C5 is sufficient for C5 inhibition. This product is NOT FOR THERAPEUTIC USE. View a summary of supporting anti-eculizumab antibody products |
- Target Species
- Human
- Product Form
- Human IgG2/4 (kappa) antibody - liquid
- Preparation
- Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Human C5
- Purity
- >98% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Eculizumab | HCA312 | E | 0.1 mg |
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| Description | Human anti Eculizumab | ||||||
| Human anti Eculizumab | HCA313 | E | 0.1 mg |
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| Description | Human anti Eculizumab | ||||||
| Human anti Eculizumab:HRP | HCA313P | E | 0.1 mg |
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| Description | Human anti Eculizumab:HRP | ||||||
| Human anti Eculizumab | HCA314 | E | 0.1 mg |
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| Description | Human anti Eculizumab | ||||||
| Human anti Eculizumab | HCA315 | E | 0.1 mg |
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| Description | Human anti Eculizumab | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK001P | CJ | 1 Conjugation For 400µg Antibody | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
MCA6145
157942
163316
165077
169456
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