C5 antibody | h5G1.1

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Human anti C5 (Eculizumab Biosimilar)

Anti-C5 Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug eculizumab (Soliris) for research use. It can be used in bioanalytical assays and to assess biological processes affected by the drug.

Product Type
Monoclonal Antibody
Clone
h5G1.1
Isotype
HuCAL IgG2
HuCAL IgG4
Product Code Applications Pack Size List Price Quantity
MCA6145
Datasheet
E
SDS
0.2 mg loader

Human Anti-C5 alpha antibody, clone h5G1.1, is a research grade biosimilar of the monoclonal antibody drug eculizumab. It is a recombinant human IgG2/4 kappa antibody with variable regions from the therapeutic eculizumab antibody clone h5G1.1. This product is supplied in a research grade format, i.e. in PBS buffer with preservative; it is not manufactured or formulated in the same way as the therapeutic reference product.

Human Anti-C5 Antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-eculizumab antibodies for example HCA312 and HCA313.

Eculizumab (Soliris) is a humanized IgG2/4 kappa antibody. The heavy chains are comprised of human IgG2 sequences in CH1, the hinge and the adjacent portion CH2, and human IgG4 sequences in the remaining part of CH2 and CH3. The drug is used to treat paroxysmal nocturnal hemoglobinuria (PNH), a rare acquired life-threatening disease of the blood characterized by destruction of red blood cells by the complement system. It also has approval as an orphan drug for treatment of atypical hemolytic uremic syndrome (aHUS). Eculizumab specifically binds to the terminal complement component 5 (C5) and inhibits the cleavage of C5 to C5a and C5b by the C5 convertase, which prevents the generation of the terminal complement complex C5b-9. Both C5a and C5b-9 cause the terminal complement-mediated events that are characteristic of PNH and aHUS. A 1:1 molar ratio of eculizumab to C5 is sufficient for C5 inhibition.

This product is NOT FOR THERAPEUTIC USE.

View a summary of supporting anti-eculizumab antibody products

Product Details

Target Species
Human
Product Form
Human IgG2/4 (kappa) antibody - liquid
Preparation
Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Human C5
Purity
>98% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
Licensed Use
For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
Regulatory
For research purposes only

Applications of C5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Eculizumab HCA312 E 0.1 mg loader
Human anti Eculizumab HCA313 E 0.1 mg loader
Human anti Eculizumab:HRP HCA313P E 0.1 mg loader
Human anti Eculizumab HCA314 E 0.1 mg loader
Human anti Eculizumab HCA315 E 0.1 mg loader
LYNX Rapid HRP Antibody Conjugation Kit LNK001P CJ 1 Conjugation For 400µg Antibody

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