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BCLAF1 antibody | AB02/2F2

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siRNA Validated
IP-MS Validated
Anti BCLAF1 Antibody, clone AB02/2F2 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti BCLAF1 Antibody, clone AB02/2F2 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti BCLAF1 Antibody, clone AB02/2F2 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti BCLAF1 Antibody, clone AB02/2F2 (PrecisionAb Monoclonal Antibody) thumbnail image 4
Anti BCLAF1 Antibody, clone AB02/2F2 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with BCLAF1 antibody (1/500, VMA00910) followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to BCLAF1 (molecular weight 151 kDa)
Anti BCLAF1 Antibody, clone AB02/2F2 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of Mouse anti BCLAF1 from HEK293 whole cell lysate. The Input lane is 6% input; the Mouse anti BCLAF1-IP lane is the immunoprecipitation with the Mouse anti BCLAF1 antibody (2.5 µg, VMA00910); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA691). Western blot analysis was performed using Mouse anti BCLAF1 antibody (1/500, VMA00910) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10000, , 1705047) and visualized on the ChemiDoc MP. Arrow points to Mouse anti BCLAF1.
Anti BCLAF1 Antibody, clone AB02/2F2 (PrecisionAb Monoclonal Antibody) gallery image 3
Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against BCLAF1 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti BCLAF1 antibody (VMA00910) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 109 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.
Anti BCLAF1 Antibody, clone AB02/2F2 (PrecisionAb Monoclonal Antibody) gallery image 4
Normalized signal intensity of BCLAF1 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against BCLAF1 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

Mouse anti BCLAF1

Product Type
PrecisionAb Monoclonal
Clone
AB02/2F2
Isotype
IgG1
Format
Purified
Specificity
BCLAF1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00910
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SDS Safety Datasheet SDS
IP WB 100 µl loader
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Mouse anti BCLAF1 antibody recognizes Bcl-2-associated transcription factor 1, also known as BCLF1.

BCLAF1 is a transcription factor involved with multiple processes including apoptosis, T-cell activation, lung development, and angiogenesis (Wen et al. 2018). BCLAF1 has been reported to act as both a tumor suppressor and an oncogene, and so its role in carcinogenesis is not clear, but aberrant alternative splicing of BCLAF1 pre-mRNA appears to be a major contributor to colon cancer development (Zhou et al. 2014). BCLAF1 may play a role in establishing senescence in response to DNA damage (Shao et al. 2016). Recently, a function of BCLAF1 in antiviral immunity has been suggested through IFN signaling (Qin et al. 2019).		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti BCLAF1 detects a band of approximately 151 kDa in HEK293 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 501-920 of human BCLAF1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/500 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q9NYF8
Entrez Gene
BCLAF1
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0005737 cytoplasm
GO:0005730 nucleolus
GO:0006350 transcription
GO:0006917 induction of apoptosis
GO:0016564 transcription repressor activity

VMA00910

100005614

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