ATP5O antibody | OTI2E9

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Anti ATP5O Antibody, clone OTI2E9 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti ATP5O Antibody, clone OTI2E9 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti ATP5O Antibody, clone OTI2E9 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti ATP5O Antibody, clone OTI2E9 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human ATP5O antibody, clone OTI2E9 (**VMA00215**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 96 second exposure. Arrow points to ATP5O (molecular weight 23 kDa).

Anti ATP5O Antibody, clone OTI2E9 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against ATP5O (1 & 2) or a scrambled siRNA (-) probed with Mouse anti ATP5O antibody (**VMA00215**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 132 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.

Anti ATP5O Antibody, clone OTI2E9 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of ATP5O from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against ATP5O (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Mouse anti ATP5O

Product Type: PrecisionAb Monoclonal
Clone: OTI2E9
Isotype: IgG1
Format: Purified
Specificity: ATP5O

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00215 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human ATP5O antibody, clone OTI2E9 recognizes the ATP synthase subunit O, mitochondrial, also known as ATP synthase subunit O, mitochondrial, human ATP synthase OSCP subunit, oligomycin sensitivity conferral protein and oligomycin sensitivity conferring protein.

The protein encoded by ATP5O gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance (provided by RefSeq, Jul 2008).

Mouse anti Human ATP5O antibody, clone OTI2E9 detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti ATP5O detects a band of approximately 23 kDa in HEK293 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein corresponding to amino acids 24-213 of human ATP5O (NP_001688) produced in E. coli
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P48047
Entrez Gene: ATP5O
GO Terms: GO:0005886 plasma membrane; GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism; GO:0005753 mitochondrial proton-transporting ATP synthase complex; GO:0022904 respiratory electron transport chain; GO:0006200 ATP catabolic process; GO:0008144 drug binding; GO:0042776 mitochondrial ATP synthesis coupled proton transport; GO:0045261 proton-transporting ATP synthase complex, catalytic core F(1)