ATP5O antibody | OTI2E9
Mouse anti ATP5O
ATP5O antibody recognizes the ATP synthase subunit O, mitochondrial, also known as human ATP synthase OSCP subunit or oligomycin sensitivity conferring protein. This antibody has been extensively validated for western blotting using whole cell lysates.
ATP5O Antibody with Control Lysate
ATP5O antibody recognizes the ATP synthase subunit O, mitochondrial, also known as human ATP synthase OSCP subunit or oligomycin sensitivity conferring protein. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI2E9
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00215 | WB | 100 µl | ||||
| VMA00215KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human ATP5O antibody recognizes the ATP synthase subunit O, mitochondrial, also known as ATP synthase subunit O, mitochondrial, human ATP synthase OSCP subunit, oligomycin sensitivity conferral protein and oligomycin sensitivity conferring protein.
The protein encoded by ATP5O gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance (provided by RefSeq, Jul 2008).
Mouse anti Human ATP5O antibody detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by ATP5O gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance (provided by RefSeq, Jul 2008).
Mouse anti Human ATP5O antibody detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti ATP5O detects a band of approximately 23 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant protein corresponding to amino acids 24-213 of human ATP5O (NP_001688) produced in E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P48047 Related reagents
- Entrez Gene
- ATP5O Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism
- GO:0005753 mitochondrial proton-transporting ATP synthase complex
- GO:0022904 respiratory electron transport chain
- GO:0006200 ATP catabolic process
- GO:0008144 drug binding
- GO:0042776 mitochondrial ATP synthesis coupled proton transport
- GO:0045261 proton-transporting ATP synthase complex, catalytic core F(1)
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of ATP5O antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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