ATP5O antibody | OTI2E9

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Anti ATP5O Antibody, clone OTI2E9 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with ATP5O antibody (**VMA00215**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 96 second exposure. Arrow points to ATP5O (molecular weight 23 kDa).

Mouse anti ATP5O

ATP5O antibody recognizes the ATP synthase subunit O, mitochondrial, also known as human ATP synthase OSCP subunit or oligomycin sensitivity conferring protein. This antibody has been extensively validated for western blotting using whole cell lysates.

ATP5O Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI2E9
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00215	WB	100 µl
VMA00215KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human ATP5O antibody recognizes the ATP synthase subunit O, mitochondrial, also known as ATP synthase subunit O, mitochondrial, human ATP synthase OSCP subunit, oligomycin sensitivity conferral protein and oligomycin sensitivity conferring protein.

The protein encoded by ATP5O gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance (provided by RefSeq, Jul 2008).

Mouse anti Human ATP5O antibody detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti ATP5O detects a band of approximately 23 kDa in HEK293 cell lysates
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein corresponding to amino acids 24-213 of human ATP5O (NP_001688) produced in E. coli
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P48047 Related reagents
Entrez Gene: ATP5O Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism; GO:0005753 mitochondrial proton-transporting ATP synthase complex; GO:0022904 respiratory electron transport chain; GO:0006200 ATP catabolic process; GO:0008144 drug binding; GO:0042776 mitochondrial ATP synthesis coupled proton transport; GO:0045261 proton-transporting ATP synthase complex, catalytic core F(1)
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of ATP5O antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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