ATP5O antibody | OTI2E9

Mouse anti ATP5O

Product Type
PrecisionAb Monoclonal
Clone
OTI2E9
Isotype
IgG1
Format
Purified
Specificity
ATP5O

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VMA00215
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WB 100 µl loader
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Mouse anti Human ATP5O antibody, clone OTI2E9 recognizes the ATP synthase subunit O, mitochondrial, also known as ATP synthase subunit O, mitochondrial, human ATP synthase OSCP subunit, oligomycin sensitivity conferral protein and oligomycin sensitivity conferring protein.

The protein encoded by ATP5O gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance (provided by RefSeq, Jul 2008).

Mouse anti Human ATP5O antibody, clone OTI2E9 detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti ATP5O detects a band of approximately 23 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 24-213 of human ATP5O (NP_001688) produced in E. coli
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P48047
Entrez Gene
ATP5O
GO Terms
GO:0005886 plasma membrane
GO:0046933 hydrogen ion transporting ATP synthase activity, rotational mechanism
GO:0005753 mitochondrial proton-transporting ATP synthase complex
GO:0022904 respiratory electron transport chain
GO:0006200 ATP catabolic process
GO:0008144 drug binding
GO:0042776 mitochondrial ATP synthesis coupled proton transport
GO:0045261 proton-transporting ATP synthase complex, catalytic core F(1)
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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