ARF1 antibody | E01/8D1

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Mouse anti ARF1

Product Type
PrecisionAb Monoclonal
Clone
E01/8D1
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00931
Datasheet
WB
SDS
100 µl loader

Mouse anti ARF1 antibody recognizes ADP-ribosylation factor 1.

ARF1 is a small GTPase which cycles between an inactive, GDP-bound state and an active, GTP-bound state. When active, ARF1 can tightly bind effectors and this binding results in biological function (Beck et al. 2008). ARF1 is involved with membrane traffic at the Golgi, including vesicle budding and podocyte function (Rafiq et al. 2017). Roles of ARF1 have also been identified in connectivity of mitochondrial networks and mitophagy (Ackema et al. 2014). Overexpression of ARF1 stimulates tumor progression and invasion, and therefore, blocking its activation has been suggested as a way to inhibit cancer progression (Prieto-Dominguez et al. 2019), and also to enhance the effectiveness of chemotherapy (Xu et al. 2017).

Product Details

Target Species
Human
Western Blotting
Mouse anti ARF1 detects a band of approximately 19 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-181 of human ARF1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P84077
Entrez Gene
ARF1
GO Terms
GO:0005886 plasma membrane
GO:0000139 Golgi membrane
GO:0005515 protein binding
GO:0003924 GTPase activity
GO:0005829 cytosol
GO:0005057 receptor signaling protein activity
GO:0005525 GTP binding
GO:0006878 cellular copper ion homeostasis
GO:0006890 retrograde vesicle-mediated transport, Golgi to ER
GO:0006892 post-Golgi vesicle-mediated transport
GO:0007264 small GTPase mediated signal transduction
GO:0016032 viral reproduction
GO:0015031 protein transport
GO:0048205 COPI coating of Golgi vesicle
GO:0048471 perinuclear region of cytoplasm
GO:0050690 regulation of defense response to virus by virus
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ARF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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