ALAS1 antibody | OTI1C5

Mouse anti ALAS1

Product Type
PrecisionAb Monoclonal
Clone
OTI1C5
Isotype
IgG1
Format
Purified
Specificity
ALAS1

Product Code Applications Pack Size List Price Your Price Qty
VMA00203
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human ALAS1 antibody, clone OTI1C5 recognizes ALAS1, also known as 5-aminolevulinate synthase, nonspecific, mitochondrial, 5-aminolevulinic acid synthase 1, ALAS-H, delta-ALA synthase 1, delta-aminolevulinate synthase 1 and migration-inducing protein 4.

The ALAS1 gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. This enzyme is a housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of ALAS1 is located on chromosome 12. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Dec 2011).

Mouse anti Human ALAS1 antibody, clone OTI1C5 detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti ALAS1 detects a band of approximately 70 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 57-308 of human ALAS1 (NP_000679) produced in E. coli
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P13196
Entrez Gene
ALAS1
GO Terms
GO:0003870 5-aminolevulinate synthase activity
GO:0005759 mitochondrial matrix
GO:0006783 heme biosynthetic process
GO:0016769 transferase activity, transferring nitrogenous groups
GO:0030170 pyridoxal phosphate binding
Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products