ALAS1 antibody | OTI1C5
siRNA Validated
Mouse anti ALAS1
- Product Type
- PrecisionAb Monoclonal
- Clone
- OTI1C5
- Isotype
- IgG1
- Format
- Purified
- Specificity
- ALAS1
Antibody Quality and Performance Guarantee
| Mouse anti Human ALAS1 antibody, clone OTI1C5 recognizes ALAS1, also known as 5-aminolevulinate synthase, nonspecific, mitochondrial, 5-aminolevulinic acid synthase 1, ALAS-H, delta-ALA synthase 1, delta-aminolevulinate synthase 1 and migration-inducing protein 4. The ALAS1 gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. This enzyme is a housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of ALAS1 is located on chromosome 12. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Dec 2011). Mouse anti Human ALAS1 antibody, clone OTI1C5 detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
|
- Target Species
- Human
- Western Blotting
- Anti ALAS1 detects a band of approximately 70 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant protein corresponding to amino acids 57-308 of human ALAS1 (NP_000679) produced in E. coli
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- P13196
- Entrez Gene
- ALAS1
- GO Terms
- GO:0003870 5-aminolevulinate synthase activity
- GO:0005759 mitochondrial matrix
- GO:0006783 heme biosynthetic process
- GO:0016769 transferase activity, transferring nitrogenous groups
- GO:0030170 pyridoxal phosphate binding
VMA00203
167116
240615
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up