ALAS1 antibody | OTI1C5

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Mouse anti ALAS1

ALAS1 antibody recognizes 5-aminolevulinate synthase, nonspecific, mitochondrial, also known as 5-aminolevulinic acid synthase 1, ALAS-H, delta-ALA synthase 1 or migration-inducing protein 4. This antibody has been extensively validated for western blotting using whole cell lysates.

ALAS1 Antibody with Control Lysate

ALAS1 antibody recognizes 5-aminolevulinate synthase, nonspecific, mitochondrial, also known as 5-aminolevulinic acid synthase 1, ALAS-H, delta-ALA synthase 1 or migration-inducing protein 4. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1C5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00203 WB 100 µl
VMA00203KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human ALAS1 antibody recognizes ALAS1, also known as 5-aminolevulinate synthase, nonspecific, mitochondrial, 5-aminolevulinic acid synthase 1, ALAS-H, delta-ALA synthase 1, delta-aminolevulinate synthase 1 and migration-inducing protein 4.

The ALAS1 gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. This enzyme is a housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of ALAS1 is located on chromosome 12. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Dec 2011).

Mouse anti Human ALAS1 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 57-308 of human ALAS1 (NP_000679) produced in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P13196 Related reagents
Entrez Gene
ALAS1 Related reagents
GO Terms
GO:0003870 5-aminolevulinate synthase activity
GO:0005759 mitochondrial matrix
GO:0006783 heme biosynthetic process
GO:0016769 transferase activity, transferring nitrogenous groups
GO:0030170 pyridoxal phosphate binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ALAS1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti ALAS1 detects a band of approximately 70 kDa in Jurkat cell lysates.
Western Blotting
Anti ALAS1 detects a band of approximately 70 kDa in Jurkat cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB