ALAS1 antibody | OTI1C5
Mouse anti ALAS1
ALAS1 antibody recognizes 5-aminolevulinate synthase, nonspecific, mitochondrial, also known as 5-aminolevulinic acid synthase 1, ALAS-H, delta-ALA synthase 1 or migration-inducing protein 4. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
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The ALAS1 gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. This enzyme is a housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of ALAS1 is located on chromosome 12. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Dec 2011).
Mouse anti Human ALAS1 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti ALAS1 detects a band of approximately 70 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- Recombinant protein corresponding to amino acids 57-308 of human ALAS1 (NP_000679) produced in E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- P13196 Related reagents
- Entrez Gene
- ALAS1 Related reagents
- GO Terms
- GO:0003870 5-aminolevulinate synthase activity
- GO:0005759 mitochondrial matrix
- GO:0006783 heme biosynthetic process
- GO:0016769 transferase activity, transferring nitrogenous groups
- GO:0030170 pyridoxal phosphate binding
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of ALAS1 antibody
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PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||WB||2 ml|
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