ACTR2 antibody | CD01/1A6

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Mouse anti ACTR2

Product Type
PrecisionAb Monoclonal
Clone
CD01/1A6
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00894
Datasheet
WB
SDS
100 µl loader

Mouse anti ACTR2 antibody recognizes actin-related protein 2, also known as ARP2.

Arp2 and Arp3 are part of the Arp2/3 complex, a multiprotein complex capable of generating branched actin networks. Actin cytoskeleton branching is essential for efficient cell migration, and silencing almost any subunit of the Arp2/3 complex has been shown to lead to a significant decrease in cell migratory capability (Rauhala et al. 2013). When activated, Arp2 and Arp3 are brought together within the complex. The active complex cross-links polymerizing actin filaments and exerts a pushing force (Molinie and Gautreau, 2018). Phosphorylation of Arp2 by NIK is required for the activity of the Arp2/3 complex (LeClaire et al. 2015).

Product Details

Target Species
Human
Western Blotting
Mouse anti ACTR2 antibody detects a band of approximately 40 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-394 of human ACTR2
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P61160
Entrez Gene
ACTR2
GO Terms
GO:0003779 actin binding
GO:0005524 ATP binding
GO:0005737 cytoplasm
GO:0005885 Arp2/3 protein complex
GO:0006928 cellular component movement
GO:0042995 cell projection
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ACTR2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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