Actin Beta antibody

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Mouse anti Actin Beta

Actin beta antibody recognizes cytoplasmic actin beta, also known as beta-actin or cytoplasmic 1. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00048
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human actin beta antibody recognizes cytoplasmic actin beta, a 375 amino acid cytoskeletal protein known also as ACTB and beta actin.

Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals (Vandekerckhove et al. 1978). Mouse anti Human actin beta antibody is highly specific for cytoplasmic actin beta and does not react with other actin isoforms.

Mouse anti Human actin beta antibody recognizes actin beta as a single band of ~42 kDa in a wide range of cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti beta actin antibody detects a band of approximately 42 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the acetylated N-terminal nonapeptide of beta-cytoplasmic actin coupled to KLH
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P60709 Related reagents
Entrez Gene
ACTB Related reagents
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Actin Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Product Specific References

References for Actin Beta antibody

  1. Sadewasser,A. et al. (2019) Anti-Niemann Pick C1 Single-Stranded Oligonucleotides with Locked Nucleic Acid Potently Reduce Ebola Virus Infection In Vitro
    Molecular Therapy - Nucleic Acids. [Epub ahead of print].

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