CD8 antibody | CVS8

Mouse anti Horse CD8

Product Type
Monoclonal Antibody
Clone
CVS8
Isotype
IgG1
Specificity
CD8

Product Code Applications Pack Size List Price Your Price Qty
MCA2385GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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Mouse anti Horse CD8 (clone CVS8), is a monoclonal antibody recognising the equine homologue of the human CD8 cell surface antigen which is expressed by a subset of T lymphocytes.

A study undertaken using CVS8 to identify CD8 on several wild african equid species indicates that the CVS8 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra) (Ibrahim  2007).

Target Species
Horse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Ass
Zebra
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Equine PBMCs.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD8 antibody

  1. Cequier, A. et al. (2022) Equine Mesenchymal Stem Cells Influence the Proliferative Response of Lymphocytes: Effect of Inflammation, Differentiation and MHC-Compatibility.
    Animals (Basel). 12 (8): 984.
  2. Lunn, D.P et al (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets
    Immunology 74: 251-257.
  3. Jacks, S. (2007) Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction.
    Clin Vaccine Immunol. 14: 669-77.
  4. Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses.
    Can J Vet Res. 71: 213-7.
  5. Lunn, D.P. et al. (1998) Report of the second equine leucocyte antigen workshop, Squaw Valley, California July 1995.
    Vet Immunol Immunopathol. 62: 101-143.
  6. Merant, C. et al. (2003) Cross-species reactivity of seven monoclonal antibodies with equine lymphocytes by flow cytometry.
    Vet Res. 34: 791-801.
  7. Ibrahim, S (2007) Analysis of monoclonal antibody cross-reactivity with leukocytes from equids and cloning of CD28
    Chapter 5 in PhD Thesis Freie Universität Berlin
  8. Carossino, M. et al. (2019) Equine arteritis virus long-term persistence is orchestrated by CD8+ T lymphocyte transcription factors, inhibitory receptors, and the CXCL16/CXCR6 axis.
    PLoS Pathog. 15 (7): e1007950.
  9. View The Latest Product References
  10. Tomlinson, J.E. et al. (2018) Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood.
    Vet Immunol Immunopathol. 199: 22-31.
  11. Placci, M. et al. (2020) Natural Horse Boarding Vs Traditional Stable: A Comparison of Hormonal, Hematological and Immunological Parameters.
    J Appl Anim Welf Sci. 23 (3): 366-77.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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