CD8 antibody | CVS8
Mouse anti Equine CD8 antibody, clone CVS8 (MCA2385GA) used for the demonstration of CD8 expressing T lymphocytes in ampullae of virus infected horses by immunohistochemistry of formalin fixed, paraffin embedded tissue sections.
Image caption:
Inflammatory response in the ampullae during long-term EAV persistence.
(B) The inflammatory infiltration is characterized by the predominance of CD8+ T lymphocytes. CD8-specific immunostaining. DAB. 100X. Bar = 80 μm.
From: Carossino M, Dini P, Kalbfleisch TS, Loynachan AT, Canisso IF, Cook RF, et al. (2019)
Equine arteritis virus long-term persistence is orchestrated by CD8+ T lymphocyte transcription factors, inhibitory receptors, and the CXCL16/CXCR6 axis.
PLoS Pathog 15(7): e1007950.
DOI: 10.1371/journal.ppat.1007950.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Equine CD8 antibody, clone CVS8 (MCA2385GA) used for the demonstration of CD8 expressing T lymphocytes in ampullae of virus infected horses by immunofluorescence of formalin fixed, paraffin embedded tissue sections.
Image caption:
Inflammatory response in the ampullae during long-term EAV persistence.
Inflammatory response in the ampullae during long-term EAV persistence.
(C) The lymphocytic infiltration was also closely associated with the luminal epithelium, with presence of intra- and subepithelial CD8+ T lymphocytes (inset [arrowheads], CD8-specific immunofluorescence [green]). H&E. 400X. Bar = 20 μm..
From: Carossino M, Dini P, Kalbfleisch TS, Loynachan AT, Canisso IF, Cook RF, et al. (2019)
Equine arteritis virus long-term persistence is orchestrated by CD8+ T lymphocyte transcription factors, inhibitory receptors, and the CXCL16/CXCR6 axis.
PLoS Pathog 15(7): e1007950.
https://doi.org/10.1371/journal.ppat.1007950.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Horse CD8
- Product Type
- Monoclonal Antibody
- Clone
- CVS8
- Isotype
- IgG1
- Specificity
- CD8
Antibody Quality and Performance Guarantee
| Mouse anti Horse CD8, clone CVS8 is a monoclonal antibody recognising the equine homologue of the human CD8 cell surface antigen which is expressed by a subset of T lymphocytes. A study undertaken using CVS8 to identify CD8 on several wild african equid species indicates that the CVS8 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra) (Ibrahim 2007). |
- Target Species
- Horse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Ass Zebra - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Equine PBMCs.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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References for CD8 antibody
-
Lunn, D.P et al (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets
Immunology 74: 251-257. -
Lunn, D.P. et al. (1998) Report of the second equine leucocyte antigen workshop, Squaw Valley, California July 1995.
Vet Immunol Immunopathol. 62: 101-143. -
Merant, C. et al. (2003) Cross-species reactivity of seven monoclonal antibodies with equine lymphocytes by flow cytometry.
Vet Res. 34: 791-801. -
Ibrahim, S (2007) Analysis of monoclonal antibody cross-reactivity with leukocytes from equids and cloning of CD28
Chapter 5 in PhD Thesis Freie Universität Berlin -
Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses.
Can J Vet Res. 71: 213-7. -
Jacks, S.
(2007) Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction.
Clin Vaccine Immunol. 14: 669-77. -
Tomlinson, J.E. et al. (2018) Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood.
Vet Immunol Immunopathol. 199: 22-31. -
Carossino, M. et al. (2019) Equine arteritis virus long-term persistence is orchestrated by CD8+ T lymphocyte transcription factors, inhibitory receptors, and the CXCL16/CXCR6 axis.
PLoS Pathog. 15 (7): e1007950.
View The Latest Product References
-
Placci, M. et al. (2020) Natural Horse Boarding Vs Traditional Stable: A Comparison of Hormonal, Hematological and Immunological Parameters.
J Appl Anim Welf Sci. 23 (3): 366-77. -
Cequier, A. et al. (2022) Equine Mesenchymal Stem Cells Influence the Proliferative Response of Lymphocytes: Effect of Inflammation, Differentiation and MHC-Compatibility.
Animals (Basel). 12 (8): 984. -
Siqueira, R.F. & Fernandes, R.L. (2018) Cryopreservation of lymphocytes for immunological studies in horses
Pesquisa Veterinária Brasileira. 38 (11): 2019-22. -
Kamm, J.L. et al. (2021) Immune response to allogeneic equine mesenchymal stromal cells.
Stem Cell Res Ther. 12 (1): 570.
MCA2385GA
148203
166446
1704
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