CD4 antibody | CVS4

Mouse anti Horse CD4:RPE

Product Type
Monoclonal Antibody
Clone
CVS4
Isotype
IgG1
Specificity
CD4

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MCA1078PE
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Mouse anti Horse CD4 antibody, clone CVS4 recognizes Equine CD4, a ~58 kDa cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes. As in humans, equine CD4 expression is mutually exclusive with CD8 expression on mature T-cells

A study undertaken using Mouse anti Horse CD4, clone CVS4 to identify CD4 on several wild african equid species indicates that the CVS4 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra).

In addition to the CVS4 clone, other CVS clones recognising equine cell surface and MHC antigen are available from Bio-Rad.

Target Species
Horse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Equine thymocytes.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
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  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Horse CD8:FITC MCA2385F F 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Horse CD8:FITC

References for CD4 antibody

  1. Hamza, E. et al. (2011) Equine CD4(+) CD25(high) T cells exhibit regulatory activity by close contact and cytokine-dependent mechanisms in vitro.
    Immunology. 134 (3): 292-304.
  2. Lunn, D.P. et al. (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets.
    Immunology. 74 (2): 251-7.
  3. Kydd, J. et al. (1994) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991.
    Vet Immunol Immunopathol. 42 (1): 3-60.
  4. Deeg,C.A. et al. (2004) The uveitogenic potential of retinal S-antigen in horses.
    Invest Ophthalmol Vis Sci. 45: 2286-92
  5. Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses.
    Can J Vet Res. 71: 213-7.
  6. Brault, S.A. et al. (2010) The immune response of foals to natural infection with equid herpesvirus-2 and its association with febrile illness.
    Vet Immunol Immunopathol. 137: 136-41.
  7. Goodman, L.B. et al. (2007) A point mutation in a herpesvirus polymerase determines neuropathogenicity.
    PLoS Pathog. 3(11):e160.
  8. Hamza, E.et al. (2012) CD4+CD25+ T cells expressing FoxP3 in Icelandic horses affected with insect bite hypersensitivity.
    Vet Immunol Immunopathol. 148 (1-2): 139-44.
  9. View The Latest Product References
  10. Go, Y.Y. et al. (2010) Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes.
    J Virol. 84: 4898-911
  11. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
    Vet Immunol Immunopathol. 62: 101-143
  12. Ibrahim, S. et al. (2007) Screening of anti-human leukocyte monoclonal antibodies for reactivity with equine leukocytes.
    Vet Immunol Immunopathol. 119 (1-2): 63-80.
  13. Lai SW et al. (2004) Influence of Ganoderma lucidum on blood biochemistry and immunocompetence in horses.
    Am J Chin Med. 32 (6): 931-40.
  14. Ferreira-Dias, G. et al. (2005) Seasonal reproduction in the mare: possible role of plasma leptin, body weight and immune status.
    Domest Anim Endocrinol. 29 (1): 203-13.
  15. Agrícola, R. et al. (2008) Blood lymphocyte subpopulations, neutrophil phagocytosis and proteinogram during late pregnancy and postpartum in mares.
    Reprod Domest Anim. 43 (2): 212-7.
  16. de Bruijn, C.M. et al. (2007) Clinical, histopathological and immunophenotypical findings in five horses with cutaneous malignant lymphoma.
    Res Vet Sci. 83 (1): 63-72.
  17. Roberto Da Costa, R.P. et al. (2003) Peripheral blood neutrophil function and lymphocyte subpopulations in cycling mares.
    Reprod Domest Anim. 38 (6): 464-9.
  18. Uner, A. G. et al. (2013) Blood Levels of Selected Metabolic Factors, Cytokines, and Lymphocyte Subpopulations in Arabian and Thoroughbred Horses During the Longest and Shortest Days of the Year
    J Equine Vet Sci. 33 (11): 969-976.
  19. Garcia-Tapia, D. et al. (2006) Replication of West Nile virus in equine peripheral blood mononuclear cells.
    Vet Immunol Immunopathol. 110 (3-4): 229-44.
  20. Tessier, L. et al. (2015) Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells.
    PLoS One. 10 (4): e0122954.
  21. Khol-Parisini, A. et al. (2012) Highly deoxynivalenol contaminated oats and immune function in horses.
    Arch Anim Nutr. 66 (2): 149-61.
  22. Ziegler, A. et al. (2016) Identification and characterization of equine blood plasmacytoid dendritic cells.
    Dev Comp Immunol. 65: 352-7.
  23. Behrens, N.E. & Gershwin, L.J. (2015) Immune modulation of T regulatory cells and IgE responses in horses vaccinated with West Nile virus vaccine combined with a CpG ODN.
    Vaccine. 33 (43): 5764-71.
  24. Degroote, R.L. et al. (2017) Formin like 1 expression is increased on CD4+ T lymphocytes in spontaneous autoimmune uveitis.
    J Proteomics. 154: 102-108.
  25. Krakowski. L. et al. (2017) Changes in Blood Lymphocyte Subpopulations and Expression of MHC-II Molecules in Wild Mares Before and After Parturition.
    J Vet Res. 61 (2): 217-21.
  26. Witonsky, S. et al. (2019) Can levamisole upregulate the equine cell-mediated macrophage (M1) dendritic cell (DC1) T-helper 1 (CD4 Th1) T-cytotoxic (CD8) immune response in vitro.?
    J Vet Intern Med. 33 (2): 889-96.
  27. Marteles, D. et al. (2019) Effects of allergen-specific immunotherapy on peripheral blood regulatory T cells and serum concentrations of cytokines and immunoglobulins in horses with allergic dermatitis.
    Int Immunopharmacol. 74: 105674.
  28. Hillmann, A. et al. (2019) A novel direct co-culture assay analyzed by multicolor flow cytometry reveals context- and cell type-specific immunomodulatory effects of equine mesenchymal stromal cells.
    PLoS One. 14 (6): e0218949.
  29. Tomlinson, J.E. et al. (2018) Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood.
    Vet Immunol Immunopathol. 199: 22-31.
  30. Placci, M. et al. (2020) Natural Horse Boarding Vs Traditional Stable: A Comparison of Hormonal, Hematological and Immunological Parameters.
    J Appl Anim Welf Sci. 23 (3): 366-77.
  31. Lucassen, A. et al. (2021) A Saccharomyces cerevisiae Fermentation Product (Olimond BB) Alters the Early Response after Influenza Vaccination in Racehorses.
    Animals (Basel). 11(9):2726.
  32. Schauer, M. et al. (2018) Interaction of septin 7 and DOCK8 in equine lymphocytes reveals novel insights into signaling pathways associated with autoimmunity.
    Sci Rep. 8 (1): 12332.
  33. Cequier, A. et al. (2022) Equine Mesenchymal Stem Cells Influence the Proliferative Response of Lymphocytes: Effect of Inflammation, Differentiation and MHC-Compatibility.
    Animals (Basel). 12 (8): 984.

Flow Cytometry

UniProt
F6Y6X8
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