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CD2 antibody | Mac288

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Anti Horse CD2 Antibody, clone Mac288 thumbnail image 1 Anti Horse CD2 Antibody, clone Mac288 thumbnail image 2
Anti Horse CD2 Antibody, clone Mac288 gallery image 1
Staining of equine peripheral blood lymphocytes with Rat anti Equine CD2 (MCA1278), visualized with Rabbit anti Rat IgG:FITC (STAR17B).
Anti Horse CD2 Antibody, clone Mac288 gallery image 2
Figure A. RPE conjugated Mouse anti Horse CD5 (MCA1079PE) and purified Mouse IgG2a isotype control (MCA929) detected with Goat anti Rat IgG2a:FITC (STAR113F). Figure B. RPE conjugated Mouse anti Horse CD5 (MCA1079PE) and Rat anti Horse CD2 (MCA1278) detected with Goat anti Rat IgG2a:FITC (STAR113F). All experiments performed on red cell lysed horse blood gated on lymphocytes in the presence of 10% horse serum. Data acquired on the ZE5™ Cell Analyzer.

Rat anti Horse CD2

Product Type
Monoclonal Antibody
Clone
Mac288
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1278
Datasheet
C F
MSDS
2 ml

Rat anti Horse CD2 antibody, clone Mac288 recognizes equine CD2, a 347 amino acid, single pass type-1 transmembrane glycoprotein containing single Ig-like C2-type and Ig-like V-type domains.

Equine CD2 is expressed by a large proportion of peripheral blood lymphocytes, most T-cells and few B-cells, it is not present on granulocytes. Immunohistochemical staining shows expression in most T cells of lymph node paracortex and occasional staining of lymphocytes in the follicular areas. In the thymus, rat anti horse CD2 antibody, clone Mac288 stains the majority of mature thymocytes in the medulla and at least half of the immature thymocytes in the cortex (Kydd et al. 1994). Rat anti Horse CD2 antibody, clone Mac288 recognizes CD2 in Haflinger and Lusitano horse breeds (Ferreira-Dias et al. 2005). Rat anti Horse CD2 antibody, clone Mac288 is a reliable pan T-cell marker for analysis of cell mediated immunity in horses infected with equine herpes virus by multi-parameter flow cytometry (Platt et al. 2010).

Product Details

Target Species
Horse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Equine peripheral blood lymphocytes.
Fusion Partners
Spleen cells from immunized LOU Rats were fused with cells of the Y3 Ag1.2.3. myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P37998 Related reagents
Entrez Gene
CD2 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
Regulatory
For research purposes only

Applications of CD2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/200
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recomended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 7.5 x 105 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader

Product Specific References

References for CD2 antibody

  1. Tavernor, A.S. et al. (1994) Expression cloning of an equine T-lymphocyte glycoprotein CD2 cDNA. Structure-based analysis of conserved sequence elements.
    Eur J Biochem. 219 (3): 969-76.
  2. Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses.
    Can J Vet Res. 71: 213-7.
  3. Kydd, J.H. (1989) Maternal immune responses to pregnancy in equids.
    PhD Thesis (Cantab).
  4. Platt, R. et al. (2010) Cell-mediated immunity evaluation in foals infected with virulent equine herpesvirus-1 by multi-parameter flow cytometry.
    Vet Immunol Immunopathol. 135: 275-81.
  5. Roberto Da Costa, R.P. et al. (2003) Peripheral blood neutrophil function and lymphocyte subpopulations in cycling mares.
    Reprod Domest Anim. 38: 464-9.
  6. McCandless, E.E. et al. (2013) Hydrocortisone inhibits IFN-γ production in equine, ovine, and bovine PBMCs.
    Vet Immunol Immunopathol. 153: 128-33.
  7. Krakowski, L. et al. (2017) Changes in blood lymphocyte subpopulations and expression of MHC-II molecules in wild mares before and after parturition
    <a href="https://www.degruyter.com/view/j/jvetres.2017.61.issue-2/jvetres-2017-0028/jvetres-2017-0028.xml"target="_blank">J Vet Res. 61 (2): 217-21.</a>
  8. Siqueira, R.F. and Fernandes, I.R. (2018) Cryopreservation of lymphocytes for immunological studies in horses
    Pesquisa Veterinária Brasileira. 38 (11): 2019-22.
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