Rat anti Horse CD2 antibody, clone Mac288
recognizes equine CD2, a 347 amino acid, single pass type-1 transmembrane glycoprotein containing single Ig-like C2-type
and Ig-like V-type
Equine CD2 is expressed by a large proportion of peripheral blood lymphocytes, most T-cells and few B-cells, it is not present on granulocytes. Immunohistochemical staining shows expression in most T cells of lymph node paracortex and occasional staining of lymphocytes in the follicular areas. In the thymus, rat anti horse CD2 antibody, clone Mac288 stains the majority of mature thymocytes in the medulla and at least half of the immature thymocytes in the cortex (Kydd et al. 1994
). Rat anti Horse CD2 antibody, clone Mac288 recognizes CD2 in Haflinger and Lusitano horse breeds (Ferreira-Dias et al. 2005
). Rat anti Horse CD2 antibody, clone Mac288 is a reliable pan T-cell marker for analysis of cell mediated immunity in horses infected with equine herpes virus by multi-parameter flow cytometry (Platt et al. 2010
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- Equine peripheral blood lymphocytes.
- Fusion Partners
- Spleen cells from immunized LOU Rats were fused with cells of the Y3 Ag1.2.3. myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD2 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recomended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 7.5 x 105 cells in 100ul.
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Secondary Antibodies Available
Product Specific References
References for CD2 antibody
Tavernor, A.S. et al. (1994) Expression cloning of an equine T-lymphocyte glycoprotein CD2 cDNA. Structure-based analysis of conserved sequence elements.
Eur J Biochem. 219 (3): 969-76.
Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses.
Can J Vet Res. 71: 213-7.
Kydd, J.H. (1989) Maternal immune responses to pregnancy in equids.
PhD Thesis (Cantab).
Platt, R. et al. (2010) Cell-mediated immunity evaluation in foals infected with virulent equine herpesvirus-1 by multi-parameter flow cytometry.
Vet Immunol Immunopathol. 135: 275-81.
Roberto Da Costa, R.P. et al. (2003) Peripheral blood neutrophil function and lymphocyte subpopulations in cycling mares.
Reprod Domest Anim. 38: 464-9.
McCandless, E.E. et al. (2013) Hydrocortisone inhibits IFN-γ production in equine, ovine, and bovine PBMCs.
Vet Immunol Immunopathol. 153: 128-33.
Krakowski, L. et al. (2017) Changes in Blood Lymphocyte Subpopulations and Expression of MHC-II Molecules in Wild Mares Before and After Parturition.
J Vet Res. 61 (2): 217-21.
Siqueira, R.F. and Fernandes, I.R. (2018) Cryopreservation of lymphocytes for immunological studies in horses
Pesquisa Veterinária Brasileira. 38 (11): 2019-22.
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