B Cell Subset antibody | MsGp10

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Mouse anti Guinea Pig B Cell Subset

Product Type
Monoclonal Antibody
Clone
MsGp10
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA567 C F 2 ml
Mouse anti Guinea pig B cell subset antibody, clone MsGp10 recognizes a target expressed on the surface of mantle zone B cells in the lymph node and on follicular dendritic cells. It detects approximately 30% of lymph node lymphocytes and 50% of Ig positive cells although it does not recognise immunoglobulin. It does not recognise T cells or thymocytes but is expressed on kidney epithelia cells and some macrophages.

Product Details

Target Species
Guinea Pig
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.2
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Guinea pig splenic dendritic cells.
Fusion Partners
Spleen cells from an immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of B Cell Subset antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Product Specific References

References for B Cell Subset antibody

  1. Wang, Y. et al. (2010) Local host response to chlamydial urethral infection in male guinea pigs.
    Infect Immun. 78: 1670-81.
  2. Lacy, H.M. et al. (2011) Essential role for neutrophils in pathogenesis and adaptive immunity in Chlamydia caviae ocular infections.
    Infect Immun. 79 (5): 1889-97.
  3. Miszczyk E et al. (2014) Antigen-specific lymphocyte proliferation as a marker of immune response in guinea pigs with sustained Helicobacter pylori infection.
    Acta Biochim Pol. 61 (2): 295-303.