B Cell Subset antibody | MsGp10
Mouse anti Guinea pig B cell subset antibody, clone MsGp10 recognizes a target expressed on the surface of mantle zone B cells in the lymph node and on follicular dendritic cells. It detects approximately 30% of lymph node lymphocytes and 50% of Ig positive cells although it does not recognise immunoglobulin. It does not recognise T cells or thymocytes but is expressed on kidney epithelia cells and some macrophages.
Product Details
- Target Species
- Guinea Pig
- Product Form
- Tissue Culture Supernatant - liquid
- Preparation
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.2
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Guinea pig splenic dendritic cells.
- Fusion Partners
- Spleen cells from an immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of B Cell Subset antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
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| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
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| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
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| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
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| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
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Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for B Cell Subset antibody
-
Wang, Y. et al. (2010) Local host response to chlamydial urethral infection in male guinea pigs.
Infect Immun. 78: 1670-81. -
Lacy, H.M. et al. (2011) Essential role for neutrophils in pathogenesis and adaptive immunity in Chlamydia caviae ocular infections.
Infect Immun. 79 (5): 1889-97. -
Miszczyk E et al. (2014) Antigen-specific lymphocyte proliferation as a marker of immune response in guinea pigs with sustained Helicobacter pylori infection.
Acta Biochim Pol. 61 (2): 295-303.
Fluorescent Spectraviewer
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