B Cell Subset antibody | MsGp10

Mouse anti Guinea Pig B Cell Subset

Product Type
Monoclonal Antibody
Clone
MsGp10
Isotype
IgG1
Specificity
B Cell Subset

Product Code Applications Pack Size List Price Your Price Qty
MCA567
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 2 ml loader
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Mouse anti Guinea pig B cell subset antibody, clone MsGp10 recognizes a target expressed on the surface of mantle zone B cells in the lymph node and on follicular dendritic cells. It detects approximately 30% of lymph node lymphocytes and 50% of Ig positive cells although it does not recognise immunoglobulin. It does not recognise T cells or thymocytes but is expressed on kidney epithelia cells and some macrophages.

Target Species
Guinea Pig
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.2
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Guinea pig splenic dendritic cells.
Fusion Partners
Spleen cells from an immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for B Cell Subset antibody

  1. Wang, Y. et al. (2010) Local host response to chlamydial urethral infection in male guinea pigs.
    Infect Immun. 78: 1670-81.
  2. Lacy, H.M. et al. (2011) Essential role for neutrophils in pathogenesis and adaptive immunity in Chlamydia caviae ocular infections.
    Infect Immun. 79 (5): 1889-97.
  3. Miszczyk E et al. (2014) Antigen-specific lymphocyte proliferation as a marker of immune response in guinea pigs with sustained Helicobacter pylori infection.
    Acta Biochim Pol. 61 (2): 295-303.

RRID
AB_321191
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Guinea Pig Products