Golimumab antibody | AbD25705

Human anti Golimumab (Drug/Target Complex)

Product Type
Monoclonal Antibody
Clone
AbD25705
Isotype
HuCAL Fab monovalent
Specificity
Golimumab
Region
DRUG/TARGET COMPLEX

Product Code Applications Pack Size List Price Your Price Qty
HCA274
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SDS Safety Datasheet SDS
E 0.1 mg loader
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Human anti golimumab (drug/target complex), clone AbD25705 is an affinity matured recombinant antibody in monovalent Fab format that specifically recognizes golimumab when in complex with its target, tumor necrosis factor alpha (TNFα). Clone AbD25705 does not recognize unbound golimumab nor free TNFα. The antibody can be used in a pharmacokinetic (PK) antigen capture format assay to measure golimumab that has been captured via immobilized human TNFα. It is ideal for bioanalytical assays to measure the level of drug or biosimilar in complex with the TNFα target.

Golimumab, marketed under the brand name Simponi®, is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.

View a summary of all anti-golimumab antibodies

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Golimumab/TNFα complex
Affinity
The monovalent intrinsic affinity of AbD25705 was measured as KD = 6 nM by real time, label free molecular interaction analysis on immobilized golimumab in complex with human TNFα.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in an indirect ELISA to detect the golimumab/TNFα complex or to detect golimumab bound to immobilized TNFα.
Protocol: PK antigen capture ELISA to measure bound drug exclusively

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Hispec Assay Diluent BUF049A E IY 50 ml
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Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
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Description Mouse anti Penta Histidine Tag:HRP

References for Golimumab antibody

  1. Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
    MAbs. 11 (1): 178-190.

Synonyms
SIMPONI®
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"