Evolocumab antibody | AbD40410

Human anti Evolocumab

Product Type
Monoclonal Antibody
Clone
AbD40410
Isotype
Fab antibody
Specificity
Evolocumab

Product Code Applications Pack Size List Price Your Price Qty
New TZA008
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Human Anti-Evolocumab Antibody, clone AbD40410, is a paratope specific, inhibitory antibody that specifically recognizes the monoclonal antibody drug evolocumab. It does not recognize the drug target, human proprotein convertase subtilisin/kexin type 9 (PCSK9), nor evolocumab in complex with PCSK9. The antibody can be used to measure free evolocumab in patient samples.

The monovalent Fab format of this antibody, clone AbD40410ad (TZA008) is tagged with a SpyTag2 at the C-terminus of the Fab antibody heavy chain, enabling the user to couple it to a SpyCatcher reagent for conversion to alternative formats in less than one hour.

View a summary of SpyCatcher products

A pair of anti-evolocumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody is recommended as the detection antibody when in the bivalent HRP conjugated format, clone AbD40410pap (TZA008P), paired with an anti-evolocumab antibody in monovalent Fab format, clone AbD40406ad (TZA007), as the capture antibody.

Evolocumab (Repatha) is a fully human IgG2 lambda antibody developed using transgenic mouse technology. It has been approved for treating selected patients with high cholesterol, and for the prevention of heart attack and stroke. The drug prevents the PCSK9 protein binding to the low density lipoprotein receptor (LDLR), thereby increasing the number of LDLRs available and lowering LDL levels.

View a summary of all evolocumab antibodies

Product Form
A monovalent human recombinant Fab (lambda light chain) in the Fab-F-Spy2-H format selected from the HuCAL® phage display library and expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Evolocumab
Affinity
The monovalent intrinsic affinity of AbD40410 was measured as KD = 5 nM by real time, label free molecular interaction analysis on immobilized evolocumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details
His-tag is a registered trademark of EMD Biosciences
Repatha is a trademark of Amgen

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD40410 can be used as the detection antibody for evolocumab in a PK bridging ELISA together with TZA007 as the capture reagent.

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
SpyTag3 Peptide BLP086 IY 1 mg loader
List Price Your Price
loader
Description SpyTag3 Peptide
Human anti Evolocumab HCA378 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Evolocumab
Human anti Evolocumab HCA376 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Evolocumab
Human anti Evolocumab HCA377 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Evolocumab
Human anti Evolocumab TZA007 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Evolocumab

Synonyms
Repatha
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
View more products with Evolocumab specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"