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Dupilumab antibody | AbD47091

Human anti Dupilumab

Product Type
Monoclonal Antibody
Clone
AbD47091
Isotype
Fab antibody
Specificity
Dupilumab

Product Code Applications Pack Size List Price Your Price Qty
TZA014
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Human Anti-Dupilumab Antibody, clone AbD47091 is a paratope specific, inhibitory anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody Dupilumab. TZA014 neither recognizes free IL-4Ra nor dupilumab in complex with IL-4Ra. Consequently, this antibody can be used to measure free dupilumab levels in serum from patients.

Anti-Dupilumab monovalent Fab antibody, TZA014, can also be converted by the user into alternative antibody formats using any of the SpyCatchers available in our catalog.

View a summary of all SpyCatcher products.

Dupilumab (Dupixent) is a human IgG4/kappa antibody inhibiting both IL‐4 and IL-13 receptors, thereby blocking these receptor signaling pathways. Dupilumab inhibits the signaling of interleukins (IL) that induce inflammatory and immunological reactions in several atopic or allergic conditions, such as eczema, allergic reaction, and rhinosinusitis.

View a summary of all anti-dupilumab antibodies.

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Dupilumab
Affinity
The monovalent intrinsic affinity of AbD47091ad was measured as KD = 0.8 nM by real time, label free molecular interaction analysis on immobilized dupilumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Dupixent is a trademark of Regeneron Pharmaceuticals, Sanofi-Aventis.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD47091ad can be used as a capture antibody for dupilumab in a bridging ELISA together with TZA015 coupled to an HRP-conjugated BiSpyCatcher2 as the detection reagent.

Protocol: PK bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
SpyTag3 Peptide BLP086 IY 1 mg loader
List Price Your Price
loader
Description SpyTag3 Peptide
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Dupilumab HCA383 E 0.1 mg
List Price Your Price
Description Human anti Dupilumab
Human anti Dupilumab HCA384 E 0.1 mg
List Price Your Price
Description Human anti Dupilumab
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit
Human anti Dupilumab TZA015 E 0.1 mg
List Price Your Price
Description Human anti Dupilumab

Synonyms
Dupixent
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

TZA014

164126

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