CD90 antibody | YKIX337.217

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Anti Dog CD90 Antibody, clone YKIX337.217 thumbnail image 1

Anti Dog CD90 Antibody, clone YKIX337.217 thumbnail image 2

Anti Dog CD90 Antibody, clone YKIX337.217 thumbnail image 3

Anti Dog CD90 Antibody, clone YKIX337.217 thumbnail image 4

Anti Dog CD90 Antibody, clone YKIX337.217 gallery image 1 — Staining of canine peripheral blood lymphocytes with Rat anti Dog CD90 (**MCA1036GA**) followed by Rabbit F(ab′)2 anti Rat IgG:FITC (STAR17B)

Anti Dog CD90 Antibody, clone YKIX337.217 gallery image 2 — Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Rat IgG2a isotype control (MCA6005) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Mouse anti Canine CD90 (**MCA1036GA**) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Anti Dog CD90 Antibody, clone YKIX337.217 gallery image 3 — Figure A. Purified Rat anti Canine CD90 (**MCA1036GA**) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649) and Mouse IgG1 FITC isotype control (MCA928F). Figure B. Purified Rat anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a PE (STAR71D649) and Mouse anti Canine CD3 FITC (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Anti Dog CD90 Antibody, clone YKIX337.217 gallery image 4 — **Published customer image:**
Mouse anti Canine CD90 antibody, clone YKIX337.217 (**MCA1036GA**) used as part of a panel of antibodies to demonstrate the phenotype of canine mesenchymal stem cells by flow cytometry together with mesenchymal markers CD9, CD44 and CD105. Negative results for lineage cell markers CD34, CD45 and STRO-1 are shown.
**Image caption:**
b. Representative histograms demonstrating positive and negative staining of marrow cMSCs from a single donor

From: Bearden RN, Huggins SS, Cummings KJ, Smith R, Gregory CA, Saunders WB.
*In-vitro* characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
Stem Cell Res Ther. 2017 Oct 3;8(1):218.

Rat anti Dog CD90

Product Type: Monoclonal Antibody
Clone: YKIX337.217
Isotype: IgG2b

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1036GA	F IP			0.1 mg
MCA1036G	F IP			0.25 mg

Rat anti Dog CD90, clone YKIX337.217 recognizes the canine Thy-1 cell surface antigen, also known as CD90. CD90 is a 20-30 kDa glycoprotein expressed by peripheral T lymphocytes, monocytes, thymocytes and vascular endothelium in dogs.

Rat anti Dog CD90, clone YKIX337.217 has been demonstrated to deplete T cells when administered in vivo (Watson et al. 1993), and to block Con A driven T cell proliferation in vitro.

Rat anti Dog CD90, clone YKIX337.217 is a useful clone for the immunophenotyping of cells and discrimination between a wide variety of lymphoproliferative disorders in dogs (Adam et al. 2009).

Product Details

Target Species

Dog

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Con A stimulated canine T cells.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

Regulatory: For research purposes only

Applications of CD90 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			1/50
Immunoprecipitation

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)	STAR131A	1 ml	C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed)	STAR131B	0.5 mg	C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800	STAR16D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC	STAR17B	1 mg	F
Rabbit F(ab')2 anti Rat IgG:HRP	STAR21B	1 mg	C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)	STAR69	0.5 ml	F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)	STAR71D649GA	0.1 mg	F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)	STAR71D800GA	0.1 mg	F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed)	STAR72	0.5 mg	C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)	STAR73	0.5 ml	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rat IgG2b Negative Control	MCA6006GA	0.1 mg	F

Application Based External Images

Immunohistology - Frozen

Product Specific References

References for CD90 antibody

Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43 (3): 137-54.
Carneiro, C.M. et al. (2007) Differential impact of metacyclic and blood trypomastigotes on parasitological, serological and phenotypic features triggered during acute Trypanosoma cruzi infection in dogs.
Acta Trop. 101: 120-9.
Kang, J.W. et al. (2008) Soluble factors-mediated immunomodulatory effects of canine adipose tissue-derived mesenchymal stem cells.
Stem Cells Dev. 17: 681-93.
Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.
Am J Trop Med Hyg. 70: 618-24.
Giunchetti, R.C. et al. (2008) Histopathology, parasite density and cell phenotypes of the popliteal lymph node in canine visceral leishmaniasis.
Vet Immunol Immunopathol. 121: 23-33.
Reis, A.B. (2005) Establishment of a microplate assay for flow cytometric assessment and it is use for the evaluation of age-related phenotypic changes in canine whole blood leukocytes.
Vet Immunol Immunopathol. 103: 173-85.
Bearden, R.N. et al. (2017) In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
Stem Cell Res Ther. 8 (1): 218.
Saulnier, N. et al. (2016) Canine Placenta: A promising potential source of highly proliferative and immunomodulatory Mesenchymal Stromal Cells?
Veterinary Immunology and Immunopathology. Feb 11 [Epub ahead of print]
Nishimura, T. et al. (2017) Feeder-independent canine induced pluripotent stem cells maintained under serum-free conditions.
Mol Reprod Dev. Feb 27. [Epub ahead of print]
Lee, J. et al. (2016) Chondrogenic potential and anti-senescence effect of hypoxia on canine adipose mesenchymal stem cells.
Vet Res Commun. 40 (1): 1-10.
Rathore, K. & Cekanova, M. (2015) Effects of environmental carcinogen benzo(a)pyrene on canine adipose-derived mesenchymal stem cells.
Res Vet Sci. 103: 34-43.
Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
Cell Prolif. 50 (6)Oct 08 [Epub ahead of print].