Denosumab antibody | AbD26781_hIgG1

Human anti Denosumab

Product Type
Monoclonal Antibody
Clone
AbD26781_hIgG1
Isotype
IgG1
Specificity
Denosumab

Product Code Applications Pack Size List Price Your Price Qty
HCA283
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E 0.1 mg loader
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Human Anti-Denosumab Antibody, clone AbD26781_hIgG1 is a non-inhibitory anti-idiotypic antibody that specifically recognizes free human monoclonal antibody drug denosumab, and the drug in complex with its target, RANKL (receptor activator of nuclear factor kappa-B ligand). The antibody does not recognize free RANKL and does not inhibit binding of denosumab to RANKL.

A pair of anti-denosumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay. This antibody clone AbD26781_hIgG1 is recommended as the detection antibody, paired with either clone AbD26295_hIgG1 (HCA280) or clone AbD26862_hIgG1 (HCA282) as capture antibody. It can also be used as the detection antibody in an antigen capture format assay. This antibody is not suitable for the development of an anti-drug antibody (ADA) assay.

Denosumab (Prolia, Xgeva) is a fully human monoclonal antibody (IgG2/kappa) for the treatment of osteoporosis, treatment-induced bone loss, bone metastases, multiple myeloma, and giant cell tumor of bone. The drug specifically binds to human RANKL a protein that acts as the primary signal to promote bone removal/resorption and prevents RANKL from activating its receptor, RANK, on the surface of osteoclasts and their precursors.

View a summary of all anti-denosumab antibodies.

Product Form
Human IgG1 antibody (kappa light chain) selected from the HuCAL phage display library and expressed in a human cell line - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Immunogen
Denosumab
Affinity
The intrinsic affinity of the monovalent form of this antibody is KD=19 nM as measured by real time, label-free molecular interaction analysis on immobilized denosumab, and as KD =17 nM on the RANKL/drug complex.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Prolia and Xgeva are trademarks of Amgen Inc.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
This antibody can be used as a detection antibody in a bridging ELISA together with HCA280 or HCA282 as capture reagents. This antibody can be used in an antigen capture ELISA. Protocol: PK bridging ELISA to measure free drug..

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References for Denosumab antibody

  1. Arthur, K.K. et al. (2012) In vitro stoichiometry of complexes between the soluble RANK ligand and the monoclonal antibody denosumab.
    Biochemistry. 51 (3): 795-806.

Synonyms
Prolia
Xgeva
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"