CD4 antibody | CACT138A

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Mouse anti Bovine CD4

Product Type
Monoclonal Antibody
Clone
CACT138A
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6081 F 0.1 mg
Mouse anti Bovine CD4, clone CACT138A, recognizes bovine CD4. CD4 regulates T cell activation and acts as an accessory protein for MHC II and TCR interaction. It is a marker for bovine T helper cells. In cattle CD4+ T cells can also be found in mediastinal haemolymph nodes, lymph nodes and parafollicular lymphatic tissue (Galeotti et al. 1993). Cattle infected with viral diarrhea virus had depleted numbers of CD4+ cells (Endsley et al. 2002).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bison
Water Buffalo
Sheep
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Concentrated tissue culture supernatant - liquid
Preparation
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
Buffer Solution
Serum free tissue culture medium containing proprietary protein free supplement
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
ConA activated bovine lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG1:RPE STAR132PE 0.1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Bovine CD8a MCA6083 0.1 mg F

Product Specific References

References for CD4 antibody

  1. Howard C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle.
    Vet Immunol Immunopathol. 27 (1-3): 21-7.
  2. Galeotti M. et al. (1993) Identification of cell types present in bovine haemolymph nodes and lymph nodes by immunostaining.
    Vet Immunol Immunopathol. 36 (4): 319-31.