CD28 antibody | CC219

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Mouse anti Bovine CD28:RPE

Product Type: Monoclonal Antibody
Clone: CC219
Isotype: IgG1
Specificity: CD28

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References (4)
Applications
Storage Information
Negative Controls
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA5779PE Datasheet SDS SDS	F	100 Test		Log in
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Anti Bovine CD28 Antibody, clone CC219 thumbnail image 1

Anti Bovine CD28 Antibody, clone CC219 thumbnail image 2

Anti Bovine CD28 Antibody, clone CC219 thumbnail image 3

Anti Bovine CD28 Antibody, clone CC219 gallery image 1 — Figure A. FITC conjugated Mouse anti Bovine CD4 (MCA1653F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Bovine CD4 (MCA1653F) and RPE conjugated Mouse anti Bovine CD28 (**MCA5779PE**). All experiments performed on red cell lysed bovine blood gated on lymphocytes in the presence of 10% bovine serum. Data acquired on the ZE5 Cell Analyzer.

Anti Bovine CD28 Antibody, clone CC219 gallery image 2 — Figure A. FITC conjugated Mouse anti Bovine CD44 (MCA2433F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Bovine CD44 (MCA2433F) and RPE conjugated Mouse anti Bovine CD28 (**MCA5779PE**). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Anti Bovine CD28 Antibody, clone CC219 gallery image 3 — Figure A. RPE conjugated Mouse anti Bovine CD28 (MCA5779PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD28 (**MCA5779PE**) and FITC conjugated Mouse anti Bovine CD44 (MCA2433F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

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Mouse anti Bovine CD28, clone CC219, recognizes the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). CD28 is involved in increased production of IL-4 and IL-10 in T cells.

Mouse anti Bovine CD28, clone CC219 is able to stimulate bovine T cells in combination with anti-bovine CD3.

Mouse anti Bovine CD28, clone CC219 has previously been published under the alternative clone designation F848EC4 (Hogg et al. 2011).

Target Species: Bovine
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution: Reconstitute with 1.0 ml distilled water
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose
Immunogen: Plasmid DNA encoding the bovine CD28 gene
Fusion Partners: Spleen cells from immunized Balb/c mice were fused with cells of the SP/2/0 myeloma cell line
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

RPE 488nm laser 496 578
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578

This product is shipped at ambient temperature.
Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

How to Use the Spectraviewer

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Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra

Description	Product Code	Applications	Pack Size	Your Price
Mouse IgG1 Negative Control:RPE	MCA928PE	F	100 Tests	Log in
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Description	Mouse IgG1 Negative Control:RPE

References for CD28 antibody

Hogg, A.E. et al. (2011) Characterization of age-related changes in bovine CD8+ T-cells.
Vet Immunol Immunopathol. 140 (1-2): 47-54.
Palomo, F.R. (2015) Study of the Effects Caused by BVDV and BHV-1 on Antigen-Presenting Cells by Means of In Vivo and In Vitro Experimental Models.
Tesis Doctoral, Facultad de Veterinaria, Universidad de Córdoba
Risalde, M.A. et al. (2020) BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV.
Comp Immunol Microbiol Infect Dis. 68: 101388.
Stabel, J.R. et al. (2022) B cell phenotypes and maturation states in cows naturally infected with Mycobacterium avium subsp. Paratuberculosis.
PLoS One. 17 (12): e0278313.

RRID: AB_11152773
UniProt: Q28071
Entrez Gene: CD28
GO Terms: GO:0016021 integral to membrane; GO:0006955 immune response; GO:0042102 positive regulation of T cell proliferation; GO:0045086 positive regulation of interleukin-2 biosynthetic process; GO:0045840 positive regulation of mitosis